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目的利用高效液相色谱法同时检测市售醒酒护肝产品中9种天然功效成分(葛根素、槲皮素、水飞蓟宾、五味子醇甲、姜黄素、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA)。方法样品经体积分数为90%的乙醇超声提取,10 000r/min离心10min,取上清液进样,经C_(18)柱用甲醇-水(用H_3PO_4调pH为2.5)进行梯度洗脱,柱温20℃,流速为0.8mL/min。色谱峰保留时间定性,外标标准曲线法定量。结果在优化的条件下,9种组分在各自的线性范围内相关系数(r)≥0.998,检出限和定量限分别为0.38~0.73mg/kg[信噪比(S/N)=3]和1.27~2.43mg/kg(S/N=10),加标回收率为88.9%~103.2%,加标样品的相对标准偏差为1.3%~3.7%。结论本研究成功建立市售保健品中多类醒酒护肝功效成分的高效液相色谱同时测定方法,能够满足市售醒酒护肝产品的常规分析和质量评价要求。
OBJECTIVE To simultaneously detect 9 natural active ingredients (puerarin, quercetin, silybin, schisandrin A, curcumin, dihydrotanshinone I, cryptotanshinone, tanshinone Ⅰ and tanshinone Ⅱ A). Methods The sample was extracted by ultrasonic with 90% ethanol and centrifuged at 10 000 r / min for 10 min. The supernatant was injected into the sample and the column was eluted with methanol - water (H_3PO_4 adjusted to pH 2.5) by C_ (18) column. The column temperature was 20 ° C and the flow rate was 0.8 mL / min. Peak retention time qualitative, external standard curve method. Results Under the optimized conditions, the correlation coefficient (r) of the nine components in the linear range was 0.998. The detection limit and the limit of quantification were 0.38-0.73 mg / kg [S / N] = 3 ] And 1.27 ~ 2.43 mg / kg (S / N = 10) respectively. The recoveries of spiked samples ranged from 88.9% to 103.2%. The relative standard deviations of the spiked samples ranged from 1.3% to 3.7%. Conclusion This study successfully established a variety of commercially available health care products sober liver protection components of the high performance liquid chromatography simultaneous determination method to meet the commercial sober liver products routine analysis and quality evaluation requirements.