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作者用酶联免疫吸附分析法(ELISA)测定了精氨琥珀酸酶(ASS)的血中浓度,获得了有价值的结果,可用于肝病诊断.特报告如下.对象与方法: 从人肝脏按saheki等的方法用精制的Ass使家兔免疫,将所得的抗血清精制为IgG,用Ishikawa等的方法在Fab片段标记过氧化物酶(POD),用所得的Fab-POD经ELISA法测定血清Ass蛋白量.标准用精制Ass.以88名健康人,11例急性肝炎、54例慢性肝炎,
The authors used the enzyme-linked immunosorbent assay (ELISA) determination of the concentration of argininuclease (ASS) in the blood, obtained valuable results, can be used for the diagnosis of liver disease.Report as follows.Purpose and method: from the human liver by In the method of Saheki et al., rabbits were immunized with purified Ass, the resulting antisera was purified as IgG, peroxidase (POD) was labeled with Fab fragment by the method of Ishikawa et al., and the obtained Fab-POD was measured by ELISA Ass protein content standard refined Ass 88 healthy people, 11 cases of acute hepatitis, 54 cases of chronic hepatitis,