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目的 探讨肿瘤坏死因子 -α(TNF- α)对骨髓基质细胞护骨素 (OPG)mRNA和蛋白质表达的影响以及TNF- α在骨代谢中的作用。方法 用TNF- α干预培养的小鼠骨髓基质MBA 1细胞 ,用RT PCR和Westernblot检测TNF- α不同剂量和不同作用时间时OPG表达变化。结果 ①MBA- 1细胞表达OPG ;②TNF -α对MBA- 1细胞OPGmRNA和OPG蛋白质表达有下调作用 ,但无剂量和时间依赖性 ;③ 17- β雌二醇 (17- βE2 )能阻断TNF α对MBA 1细胞OPG表达的下调作用 ;而吡咯二硫氨基甲酸酯 (PDTC ,一种核因子 κB抑制剂 )不能拮抗此作用。结论 TNF -α下调OPG表达。雌激素拮抗TNF- α,使MBA 1细胞OPG表达上调。TNF -α对OPG表达的影响与转录因子 - κB信息通路无关。
Objective To investigate the effects of tumor necrosis factor-α (TNF-α) on osteoprotegerin (OPG) mRNA and protein expression in bone marrow stromal cells and the role of TNF-α in bone metabolism. Methods The cultured murine bone marrow stromal MBA 1 cells were treated with TNF-α. The expression of OPG was detected by RT-PCR and Western blotting at different doses and different time of TNF-α. RESULTS ① OPG was expressed in MBA-1 cells; ② TNF-α had a down-regulation of OPG mRNA and OPG protein in MBA-1 cells, but dose- and time-dependent manner; ③ 17- β estradiol (17- βE2) On the down-regulation of OPG expression in MBA 1 cells; whereas pyrrolodithiocarbamate (PDTC, a nuclear factor κB inhibitor) failed to antagonize this effect. Conclusion TNF-α down-regulated OPG expression. Estrogen antagonizes TNF-α and up-regulates OPG expression in MBA1 cells. The effect of TNF-α on OPG expression has nothing to do with the transcription factor - κB signaling pathway.