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已知去甲肾上腺素(NA)和前列腺素E类(PGE_5)可刺激培养的大鼠正中隆起释放黄体生成素释放激素(LH-RH),本实验旨在揭示肾上腺素能受体在此反应中的作用及PG对LH-RH的刺激作用是否需要与特异性PG受体结合。 重180-200g的雄性大鼠在25℃室温条件下饲养,早8点至晚8点有光照,食物和水随意给予。早9至10时将大鼠处死,取出脑组织,在立体显微镜下用虹膜剪解剖正中隆起,解剖的组织中含有大部分正中隆起和小部分垂体柄。解剖后约25分钟,
It is known that norepinephrine (NA) and prostaglandin E (PGE_5) stimulated cultured rats to release luteinizing hormone-releasing hormone (LH-RH) in the mid-elevation bulge. This experiment aims to reveal that adrenergic receptors react here And the role of PG in stimulating LH-RH need to bind to specific PG receptors. Male rats weighing 180-200 g were housed at 25 ° C at room temperature with light as early as 8 o’clock to 8 o’clock and food and water were given at random. As early as 9-10, the rats were sacrificed and the brain tissue was removed. The middle ridges were dissected with iris scissors under a stereomicroscope. The anatomical tissue contained most of the median ridges and a small portion of the pituitary stalk. About 25 minutes after dissection,