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目的:观察加味四逆散对酒精性脂肪肝大鼠血清ALT、AST及肝组织CYP2E1及TNF-α的影响。方法:将72只大鼠随机分为3组,对照组、模型组、治疗组各24只。模型组和治疗组用乙醇灌胃的方法造模,而对照组应则采用蒸馏水。治疗组在4周时灌胃酒2 h前灌胃加味四逆散1 m L/100 g,模型组灌胃等量蒸馏水。共计12周。观察各组ALT、AST及肝组织SOD、MDA及CYP2E1和TNF-α的表达。结果:与模型组比较,治疗组血清ALT、AST,肝组织CYP2E1和TNF-α的表达下降,差异有统计学意义(P<0.05),MDA水平减少(P<0.05),SOD水平升高(P<0.05)。结论:加味四逆散对大鼠酒精性脂肪肝有一定疗效,通过抑制CYP2E1和TNF-α的表达,减轻脂质过氧化反应,可能是其作用的靶点之一。
Objective: To observe the effects of Jiawei Sini Decoction on serum ALT, AST, hepatic CYP2E1 and TNF-α in alcoholic fatty liver rats. Methods: Seventy two rats were randomly divided into three groups: control group, model group and treatment group. Model group and treatment group with ethanol gavage modeling, while the control group should use distilled water. The rats in the treatment group were treated with Jia Si Si Ni San 1 m L / 100 g 2 hours prior to intragastric administration of alcohol at 4 weeks, and the model group was given the same amount of distilled water. Total 12 weeks. The ALT, AST and the expression of SOD, MDA, CYP2E1 and TNF-α in liver tissues were observed. Results: Compared with the model group, the levels of ALT, AST and the expression of CYP2E1 and TNF-α in the liver tissue of the treated group were significantly decreased (P <0.05), the level of MDA was decreased P <0.05). Conclusion: Jiawei Sini Decoction has certain curative effect on alcoholic fatty liver in rats. It may be one of its targets by inhibiting the expression of CYP2E1 and TNF-α and decreasing the lipid peroxidation.