论文部分内容阅读
矢车菊素-3-芸香糖苷(C3R)是桑椹花青素的主要成分,研究从成熟桑椹中提取C3R对链脲佐菌素(STZ)诱导人源性胰岛β细胞凋亡的抑制作用及可能的调控机制。采用MTT法及流式细胞计数检测发现:桑椹C3R对正常胰岛β细胞株INS-1具有极显著的促增殖作用(P<0.01);50μg/m L桑椹C3R液处理STZ诱导凋亡模型胰岛β细胞株INS-1 48 h后,其早期凋亡、晚期凋亡和坏死的细胞数量极显著下降(P<0.01)。以经桑椹C3R液处理的STZ诱导凋亡模型胰岛β细胞的c DNA为模板,通过qRT-PCR检测细胞中的几个凋亡相关蛋白基因的转录水平变化,其中抗凋亡蛋白基因Bcl-x L的mRNA转录水平极显著上调(P<0.01),促凋亡蛋白基因Bad、Bax及Caspase 8、Caspase 6基因的mRNA转录水平极显著下调(P<0.01)。研究结果表明,桑椹C3R对STZ诱导的胰岛β细胞凋亡具有明显的抵抗作用,由此也证实了桑椹花青素的药用开发价值。
Cyanidin-3-rutinoside (C3R) is the main component of anthocyanin. To study the inhibitory effect of C3R extracted from mature mulberry on the apoptosis of human pancreatic islet β cells induced by streptozotocin (STZ) Possible regulatory mechanisms. The results of flow cytometry and MTT assay showed that mulberry C3R had a significant effect on the proliferation of normal pancreatic β-cell line INS-1 (P <0.01), and 50μg / m L mulberry C3R treated STZ-induced apoptosis model After 48 h of INS-1, the numbers of cells in early apoptosis, late apoptosis and necrosis decreased significantly (P <0.01). The transcriptional level of several apoptosis-related protein genes was detected by qRT-PCR. The anti-apoptotic protein Bcl-x L mRNA (P <0.01). The transcriptional levels of pro-apoptotic protein Bad, Bax, Caspase 8 and Caspase 6 were significantly down-regulated (P <0.01). The results show that mulberry C3R STZ-induced pancreatic β-cell apoptosis has a significant resistance, which also confirmed the medicinal development of mulberry anthocyanin value.