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目的 :研究黄芩苷对局灶性脑缺血大鼠脑组织基因表达谱的影响 ,探索黄芩苷治疗脑缺血的药理作用机制。方法 :分别从假手术组、局灶性脑缺血组、黄芩苷治疗组SD大鼠的脑组织中抽提总RNA ,Cy3 ,Cy5荧光标记 ,反转录分别合成cDNA探针后 ,与含有 40 96条大鼠基因的BioStar基因表达谱芯片杂交 ,AxonGenepix 40 0 0B扫描仪扫描 ,GenePixPro 3 .0软件分析表达信号。结果 :大鼠局灶性脑缺血组差异表达的基因有 2 11条 ,其中 12条基因上调 ,199条基因下调。黄芩苷治疗后差异表达的基因有 177条 ,其中有 89条基因上调 ,而 88条基因下调。进一步分析发现 ,1个在模型组下调的基因经黄芩苷治疗后上调 ,3个在模型组上调的基因经黄芩苷治疗后下调 ,3个在模型组上调的基因经黄芩苷治疗后表达进一步增强。结论 :在基因组水平上黄芩苷可能通过多基因 ,多途径调节大鼠脑缺血基因表达谱而发挥药理作用
Objective : To study the effect of baicalin on gene expression profile of brain tissue in rats with focal cerebral ischemia and explore the pharmacological mechanism of baicalin in treating cerebral ischemia. METHODS: Total RNA, Cy3, and Cy5 were extracted from brain tissues of sham-operated group, focal cerebral ischemia group, and baicalin-treated SD rats, and cDNA was synthesized by reverse transcription. 40 96 rat BioStar gene expression microarray hybridizations were performed on Axon Genepix 400B scanners and GenePixPro 3.0 software was used to analyze expression signals. RESULTS: There were 21 genes that were differentially expressed in rats with focal cerebral ischemia, of which 12 genes were up-regulated and 199 genes were down-regulated. There were 177 differentially expressed genes after treatment with baicalin, of which 89 genes were up-regulated and 88 genes were down-regulated. Further analysis showed that 1 gene down-regulated in the model group was up-regulated after treatment with baicalin, 3 genes up-regulated in the model group were down-regulated after treatment with baicalin, and 3 genes up-regulated in the model group were further enhanced after treatment with baicalin. . Conclusion: Baicalin may exert its pharmacological effects at the genomic level by regulating gene expression profiles of cerebral ischemic in rats through multiple genes and pathways.