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目的研究兰州市不同地区大气细颗粒物(PM2.5)对大鼠肺泡巨噬细胞吞噬功能的影响。方法于2005年12月15日—2006年2月15日,采集兰州市西固区、铁路局、草场街、夏官营大气PM2.5样本,测定其中的Fe、Mn、Ni、Pb、Cd金属含量。分别将4个地区的大气样本配置成7.5 mg/ml的PM2.5悬液0.2 ml,通过气管注入法经呼吸道对Wistar大鼠(n=8)染毒,测定大鼠肺泡巨噬细胞的吞噬率、吞噬指数。阴性对照组(n=8)进行气管灌注生理盐水,每只0.2 ml。结果与阴性对照组比较,4个染毒组巨噬细胞吞噬功能均显著下降,差异有统计学意义(P<0.01)。4个采样点中西固区大气PM2.5中金属元素的含量最高,大鼠巨噬细胞吞噬率和吞噬指数最低,分别为(74.2±1.31)%和1.041±1.06;夏官营大气PM2.5中金属含量最少,巨噬细胞吞噬率和吞噬指数最高,分别为(81.80±1.62)%和1.593±0.23。结论兰州市不同采样点大气PM2.5对大鼠巨噬细胞的吞噬率和吞噬指数的影响有差异。大鼠巨噬细胞的吞噬功能随着PM2.5中金属元素含量的增多而下降。
Objective To study the effects of PM2.5 on phagocytosis of rat alveolar macrophages in different areas of Lanzhou City. Methods From December 15, 2005 to February 15, 2006, PM2.5 samples from Xigou, Railway Bureau, Caochang Street and Xiaguan Camp of Lanzhou City were collected and their contents of Fe, Mn, Ni, Pb, Cd Metal content. The air samples of 4 regions were respectively configured into 0.2 ml suspension of 7.5 mg / ml PM2.5 and the phagocytosis of rat alveolar macrophages was determined by airway injection into Wistar rats (n = 8) Rate, phagocytosis index. Negative control group (n = 8) were tracheal perfusion saline, 0.2 ml each. Results Compared with the negative control group, phagocytosis of macrophages in four exposure groups was significantly decreased, the difference was statistically significant (P <0.01). In the four sampling sites, the content of metal elements in PM2.5 was the highest in central and western regions, the phagocytic rate and phagocytic index of macrophages were the lowest (74.2 ± 1.31% and 1.041 ± 1.06, respectively); Xiaguan Camp PM2.5 The content of metal was the lowest, the phagocytic rate and phagocytic index of macrophages were the highest (81.80 ± 1.62)% and 1.593 ± 0.23, respectively. Conclusion The effects of atmospheric PM2.5 on the phagocytic rate and phagocytic index of macrophages in different sampling sites in Lanzhou City are different. The phagocytosis of rat macrophages decreased with the increase of metal element content in PM2.5.