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目的:研究DSPE-PEG-胆酸修饰水飞蓟宾脂质体对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法:将48只雄性ICR种小鼠随机分成6组:正常对照组、CCl_4肝损伤模型组、水飞蓟宾胶囊剂组、水飞蓟宾修饰脂质体低、中、高剂量组。采用腹腔注射CCl4致小鼠急性肝损伤模型,测定小鼠血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST);测定肝脏丙二醛(malondialdehyde,MDA)含量,谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)含量和超氧化物歧化酶(superoxide dismutase,SOD)活力,计算肝指数,并观察肝组织病理学变化。结果:与CCl_4模型组比较,各治疗组小鼠肝脏指数、血清ALT和AST活性不同程度降低(P<0.05);肝组织匀浆中MDA,GSH-Px,SOD活力不同程度升高(P<0.05);病理切片表明小鼠肝损伤不同程度减轻,而且脂质体组比胶囊剂组有较好改善。结论:DSPE-PEG-胆酸修饰水飞蓟宾脂质体对CCl_4所致小鼠急性肝损伤有显著的保护作用,且高于水飞蓟宾胶囊剂组。
Objective: To study the protective effect of DSPE-PEG-cholic acid modified silybin liposome on acute liver injury induced by carbon tetrachloride (CCl4) in mice. Methods: 48 male ICR mice were randomly divided into 6 groups: normal control group, CCl 4 liver injury model group, silybin capsule group and silybin modified liposomes in low, medium and high dose groups. Acute liver injury induced by CCl4 in mice was induced by intraperitoneal injection of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The levels of malondialdehyde (MDA), glutathione The content of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were measured to calculate the liver index, and the pathological changes of liver were observed. Results: Compared with the CCl 4 model group, the liver index and serum ALT and AST activity of mice in each treatment group decreased to different extents (P <0.05); the activity of MDA, GSH-Px and SOD in liver homogenate increased to some extent (P < 0.05). Pathological examination showed that liver damage in mice was alleviated to some extent, and the liposome group had a better improvement than the capsule group. Conclusion: DSPE-PEG-cholic acid modified silybin liposome has a significant protective effect on acute liver injury induced by CCl 4 in mice and is higher than that of silybin capsule.