采用４种抗氧化剂的不同浓度溶液，对离体稻胚作预处理，研究其抑制水稻愈伤组织褐变的效应。结果表明，每种抗氧化剂抑制愈伤组织祸变都有其最适浓度，ＰＶＰ、Ｖ_ｃ、柠檬酸和ＤＴＴ的适宜浓度分别为０．５％，０．１％，０，１％和１０ｍｇ／Ｌ或５００ｍｇ／Ｌ且各抗氧化剂抑制愈伤组织褐变的有效浓度及其有效性程度与培养期有密切的关系。作者根据研究，提出了用上述抗氧化剂防止水稻愈伤组织褐变的使用原则。 Four kinds of antioxidants in different concentrations were used to pre-treat rice embryos in vitro to study their effects on the browning of rice callus. The results showed that each antioxidant had the best concentration of callus, and the optimum concentration of PVP, Vc, citric acid and DTT was 0.5%, 0.1%, 0,1% and 10 mg / L or 500mg / L and the antioxidants inhibit callus browning effective concentration and the degree of effectiveness and culture period are closely related. According to the research, the author put forward the principle of using the above antioxidants to prevent browning of rice callus.