我院小儿科于1988年7月年3月为两例小儿急性淋巴细胞性白血病（ALL）进行骨髓移植（ABMT）治疗，其中1例应用抗CALLA型McAB加兔补体体外净化骨髓现报告如下 ：1诊断，临床及预处理方案，见附表 2.骨髓体外净化及输注使用抗CALLA型McAb_(55)加兔补体两次处理法净化骨髓。例1采集骨髓830ml,有核细胞总数为31.05×10~8,相当于病儿体重0.86×10~8/kg。净化处理后细胞回收率18.6％,置22℃～25℃室温保存52h后回输,因输注时悬液中细胞结成大块团絮状及输注反应严重,故细胞悬液再用生理盐水洗涤两次,通过四号针头形成单细胞悬液再静脉回输,未再反应,输入有核细胞数降到0.037×10~8/kg体重。因输有核细胞数量太少, Pediatrics in our hospital in July 1988 March in March for two cases of pediatric acute lymphoblastic leukemia (ALL) for bone marrow transplantation (ABMT) treatment, of which 1 case of anti-CALLA type McAB plus rabbit complement purification of bone marrow in vitro are reported as follows: 1 Diagnosis, clinical and pre-treatment programs, see Schedule 2. Purification and infusion of bone marrow in vitro anti-CALLA McAb (55) plus rabbit complement two treatments to purify the bone marrow. Example 1 collected bone marrow 830ml, the total number of nucleated cells 31.05 × 10 ~ 8, equivalent to sick children weighing 0.86 × 10 ~ 8 / kg. Purified cells recovered 18.6%, set 22 ℃ ~ 25 ℃ room temperature for 52h after transfusion, due to the infusion of cells in the formation of large groups flocculent and infusion serious, so the cell suspension and then physiology The cells were washed twice with saline and re-vented through a fourth needle to form a single cell suspension without further reaction. The number of nucleated cells decreased to 0.037 × 10 ~ 8 / kg body weight. Due to the number of transfused nucleated cells is too small,