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目的建立同时测定独一味软胶囊中山栀苷甲酯、8-O-乙酰山栀苷甲酯、芦丁、木犀草苷、毛蕊花糖苷、槲皮素等成分含量的方法。方法采用HPLC法。色谱柱为Waters Symmetry C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-水(含0.3%磷酸),梯度洗脱,流速1 m L·min-1,检测波长235 nm。结果山栀苷甲酯、8-O-乙酰山栀苷甲酯、芦丁、木犀草苷、毛蕊花糖苷、槲皮素的线性范围分别为0.67~10.68、1.29~20.65、1.36~21.8、0.65~10.46、0.69~10.98、0.13~2.08μg·m L-1。平均加样回收率分别为95.1%、93.3%、98.2%、102.3%、97.6%、101.6%,RSD分别为2.6%、4.6%、3.0%、4.0%、3.5%、4.8%(n=6)。结论所用方法专属性好、精密度高,可为独一味软胶囊的质量标准研究提供参考。
OBJECTIVE To establish a method for the simultaneous determination of the contents of atoside methyl ester, 8-O-acetylglucoside methyl ester, rutin, luteolin, verbascoside and quercetin in Douchi Soft Capsules. Methods HPLC method. The chromatographic column was a Waters Symmetry C18 column (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water with 0.3% phosphoric acid and eluted with a gradient of 1 m L · min-1 at a detection wavelength of 235 nm. Results The linear range of methyl NS, 8-O-acetylglucoside, rutin, luteolin, verbascoside and quercetin were 0.67 ~ 10.68, 1.29 ~ 20.65, 1.36 ~ 21.8 and 0.65 ~ 10.46,0.69 ~ 10.98,0.13 ~ 2.08μg · m L-1. The average recoveries were 95.1%, 93.3%, 98.2%, 102.3%, 97.6%, 101.6%, respectively. The RSDs were 2.6%, 4.6%, 3.0%, 4.0%, 3.5%, 4.8% . Conclusion The method used has good specificity and high precision, which can provide references for the research of the quality standard of the one-flavor soft capsule.