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目的 研究葡萄籽提取物原花青素诱导胃癌细胞脱落凋亡的作用。方法 采用细胞形态学观察、Annxin V和PI细胞染色实验和流式细胞仪检测方法观察原花青素对悬浮培养的胃癌细胞生长状态和细胞周期的影响以及诱导脱落凋亡的作用。结果 0 1mmol·L-1原花青素可抑制抗脱落凋亡的胃癌细胞聚集成团 ,0 5mmol·L-1原花青素可阻滞悬浮培养的不同胃癌细胞于不同的细胞周期 ,并诱导胃癌细胞发生脱落凋亡。结论 原花青素可诱导胃癌细胞发生脱落凋亡
Objective To study the effect of grape seed extract proanthocyanidin on apoptosis and apoptosis of gastric cancer cells. Methods The morphological observation, Annxin V and PI staining and flow cytometry were used to observe the effects of proanthocyanidins on the growth and cell cycle of gastric cancer cells in vitro and the role of apoptosis and apoptosis. Results 0 1mmol·L-1 proanthocyanidins could inhibit the apoptosis-resistant gastric cancer cells from aggregating into clusters, and 0 5mmol·L-1 proanthocyanidin could block different gastric cancer cell lines from suspension culture in different cell cycle and induce apoptosis of gastric cancer cells Death. Conclusion Proanthocyanidins can induce the apoptosis of gastric cancer cells