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目的观察降钙素基因相关肽(calcitonin gene related peptide,CGRP)对心脏干细胞(cardiac stem cells,CSCs)募集及心功能的影响。方法建立大鼠急性心肌梗死(AMI)模型,随机分为假手术组、CGRP注射组(CGRP组)和等体积磷酸盐缓冲液(PBS)注射组(对照组),每组30只。模型建立后于不同时间点获取标本,采用免疫荧光染色法观察CSCs募集情况,超声心动图评估大鼠心肌梗死后心功能,苏木素-伊红(H-E)染色、Masson染色评估心脏病理学改变,2,3,5-氯化三苯基四氮唑(TTC)染色测定心肌梗死面积。结果免疫荧光染色显示,与假手术组相比,对照组和CGRP组CSC细胞数均在第3天增加,7 d达高峰,14 d明显减少,其中,CGRP组较对照组增加更明显(均P<0.05)。术后28 d大鼠超声心动图检测大鼠心功能提示CGRP组较对照组心功能各项指标明显改善(均P<0.05),形态学分析显示术后28 d CGRP组心肌纤维化程度及梗死面积明显小于对照组(P<0.05)。结论CGRP可促进AMI后CSC在梗死区域募集,于第7天达高峰,并能改善大鼠心功能。
Objective To investigate the effects of calcitonin gene related peptide (CGRP) on the recruitment of cardiac stem cells (CSCs) and cardiac function. Methods Acute myocardial infarction (AMI) model of rats was established and randomly divided into sham operation group, CGRP injection group (CGRP group) and PBS injection group (control group), 30 rats in each group. After establishment of the model, samples were taken at different time points. The recruitment of CSCs was observed by immunofluorescence staining. The cardiac function was evaluated by echocardiography after myocardial infarction. The changes of cardiac pathology were assessed by HE staining and Masson staining. , 3,5- chlorotriphenyltriazole (TTC) staining myocardial infarction area. Results Immunofluorescence staining showed that the number of CSC cells in control group and CGRP group increased on the 3rd day and peaked on the 7th day, decreased significantly on the 14th day, especially in the CGRP group P <0.05). Echocardiography at 28 days after operation showed that the cardiac function of rats in CGRP group was significantly improved (all P <0.05). The morphological analysis showed that the degree of myocardial fibrosis and infarction Area was significantly smaller than the control group (P <0.05). Conclusion CGRP can promote recruitment of CSC in infarcted area after AMI, reach its peak on the 7th day and improve cardiac function in rats.