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磷矿粉中所含无机磷化合物多为难溶性磷,直接施用于石灰性土壤,不显肥效。近年来不少地区开发了大批中、低品位磷矿,用硫酸制化学磷肥耗酸量过大,少用或不用硫酸制磷肥的研究受到重视。我们结合陕西磷矿丰富而硫源缺乏的特点,探索异养型微生物对提高磷矿粉的有效性,对分离的一株F028进行了研究。 一、材料和方法 (一)菌种 于1975年分离自陕南林地腐殖质层土壤,属于Aspergillus niger群。 (二)培养基成分 根据研究目的的不同,采用不同成分的培养基。菌体发育和产酸关系试验用5％麸皮汁加1％玉米面糖化液糖。不同碳、氮源试验以缺碳、氮源的Czapek液为基础营养液,分别加入供试碳、氮源,以磷矿粉取代原来的可溶性磷盐。
Inorganic phosphorus compounds contained in phosphate rock are mostly insoluble phosphorus, directly applied to calcareous soil, no significant fertilizer efficiency. In recent years, a large number of middle and low grade phosphate rock have been developed in many areas. The use of sulfuric acid to make chemical phosphate fertilizer consumes too much acid and less or no sulfuric acid is used to make it. Based on the characteristics of abundant phosphate rock and lack of sulfur source in Shaanxi Province, we explored the effectiveness of heterotrophic microorganisms in improving phosphate rock. A F028 strain was isolated from the plant. First, the material and methods (A) strain isolated in 1975 from the southern Shaanxi humus soil, belonging to Aspergillus niger group. (B) medium composition According to the different purpose of the study, the use of different components of the medium. Cell growth and acid production test with 5% bran juice plus 1% corn flour saccharified liquid sugar. Different carbon and nitrogen sources test Czapek liquid which is lack of carbon and nitrogen source was used as the nutrient solution, and the carbon and nitrogen sources were added respectively to replace the original soluble phosphorous salt with phosphate rock powder.