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透明质酸钠(HA)是体内粘多糖的一种.分布在结缔组织之中.因其水溶液具有粘弹性,故在体内可起润滑作用,维持组织正常功能.并具有促进创伤愈合和预防细菌感染的作用.本文通过溶出试验和导电率、粘度、浊度测定,研究四环素类(TC_S)与HA间的相互作用.结果发现;(1)TC_s半数释放时间(T_5c)随HA浓度增大而延长.不同TC_S的T_5c对HA溶液浓度依赖程度不同:美他环素(methacycline.METC)释放持续时间最长.氧四环素(OTC)释放持续时间最短:(2)导电率测定结果显示.各种TC_S之间基本类似.未加入NA时.各TC_S在水溶液中缔合分子生成浓度(CMC)均在 0.32～0.36之间.表明其各目的缔合能量相近.添加 HA后,各TC_S的CMC值随HA浓度增大而增加,表明两者间可引起相互作用,各TC_S作用强度亦无显著差异;(3)在PH为2,4时加入METC后,可使HA溶液粘度增加.当HA浓度为5meq/L,pH为6时,溶液呈混浊的凝胶状态,而OTC未出现此种现象,表明METC比OTC与HA间的相互作用更强.METC释放速率依赖于pH介质的改变,而OTC无此依赖关系;(4)TCS在HA溶液中的T_(50)值与该种TC_s的表面分配系数有良好的相关性,各TC_S的亲脂性对其自身在HA溶液中的释放延缓有很大影响.
Sodium hyaluronate (HA) is a type of mucopolysaccharide distributed in the connective tissue.As its aqueous solution has viscoelasticity, it can lubricate the body and maintain the normal function of the tissue, and has the functions of promoting wound healing and preventing bacteria (TC_S) and HA were studied by dissolution test, conductivity, viscosity and turbidimetry.The results showed that: (1) The half-time release of TC_s (T_5c) Prolongation.The concentrations of T_5c in different TC_Ss were dependent on the concentration of HA solution.Metcycline.METC release duration was the longest.Oxytetracycline (OTC) release duration was the shortest: (2) The results of conductivity measurement showed that various TC_S were similar.When NA was not added, the concentration of association molecules (TCs) in each aqueous solution of TC_S was between 0.32 and 0.36, which indicated that the energy of each association was similar.When HA was added, CMC value of each TC_S With the increase of HA concentration, it shows that the interaction between the two can be caused and there is no significant difference in the intensity of each TC_S; (3) The addition of METC at pH2,4 can increase the viscosity of HA solution.When HA concentration Was 5 meq / L and the pH was 6, the solution appeared cloudy and gel state, while OTC was not This phenomenon shows that METC has a stronger interaction than OTC and HA, the release rate of METC depends on the change of pH medium, and OTC has no such dependence. (4) The value of T_ (50) The surface partition coefficient of this TC_s has a good correlation, and the lipophilicity of each TC_S has a great influence on the delay of its release in HA solution.