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老年化是心血管疾病发生的主要因素。实验研究证实:血管平滑肌细胞的增殖是动脉硬化的早期病理特征。大鼠动脉移植实验表明大鼠主动脉硬化斑块的形成以动脉本身的年龄为特征,而与实验动物自身年龄无关。因此可以设想动脉细胞损伤之后,这些细胞的高度增殖能力和老化动脉细胞自身对动脉硬化的易感性升高有关。作者分离了不同年龄组的大鼠主动脉平滑肌细胞,进行细胞培养。培养条件分为两组:一组为有血清培养(RPMIl640/199加10％胎牛血清)。另一组为无血清培养(HL-1,不含血小板来源的生长因子,但追加
Aging is a major cause of cardiovascular disease. Experimental studies confirmed that: vascular smooth muscle cell proliferation is the early pathological features of atherosclerosis. Artery transplantation in rats showed that the formation of aortic plaques in rats was characterized by the age of the arteries but not with the age of the animals themselves. It is therefore conceivable that, after arterial cell injury, the highly proliferative capacity of these cells correlates with the increased susceptibility of aging arterial cells to arteriosclerosis. The authors isolated rat aortic smooth muscle cells of different age groups for cell culture. Culture conditions were divided into two groups: a group of serum culture (RPMIl640 / 199 plus 10% fetal bovine serum). The other group was serum-free (HL-1, platelet-derived growth factor but added