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目的:研究青蒿琥酯诱导人胃癌SGC-7901细胞凋亡作用及其可能机制。方法:应用流式细胞术(FCM)、透射电镜(TEM)等方法检测不同浓度下青蒿琥酯对人胃癌SGC-7901细胞生长的影响,并应用Western Blot法检测凋亡相关基因Bcl-2、Bax的表达水平。结果:经青蒿琥酯处理后,人胃癌SGC-7901细胞凋亡率升高,并具有时间浓度依赖性(P<0.05),癌细胞被阻滞于G0/G1期;电镜观察肿瘤组织中散在凋亡细胞及凋亡小体;Western Blot法检测到凋亡相关基因Bcl-2表达减弱,Bax表达增强(P<0.05)。结论:青蒿琥酯能有效抑制人胃癌SGC-7901细胞增殖并诱导其凋亡,其机制可能与下调凋亡相关基因Bcl-2、上调Bax表达有关。
Objective: To study the apoptosis of human gastric cancer cell line SGC-7901 induced by artesunate and its possible mechanism. Methods: The effects of artesunate on the growth of human gastric cancer cell line SGC-7901 were detected by flow cytometry (FCM) and transmission electron microscopy (TEM). The apoptosis-related genes Bcl-2 , Bax expression levels. Results: After treated with artesunate, the apoptosis rate of human gastric cancer SGC-7901 cells increased in a time-dependent manner (P <0.05), and the cancer cells were arrested in G0 / G1 phase. The apoptosis of SGC- Scattered apoptotic cells and apoptotic bodies. The expression of Bcl-2 and Bax were decreased (P <0.05) by Western Blot. CONCLUSION: Artesunate can effectively inhibit the proliferation and induce the apoptosis of human gastric cancer cell line SGC-7901, which may be related to the down-regulation of Bcl-2 and up-regulation of Bax expression.