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小菜蛾(Plutella xylostella(Linn.))是十字花科蔬菜为害最重的害虫,对拟除虫菊酯类农药已经普遍产生了严重抗性。为完整了解小菜蛾对拟除虫菊酯类农药抗性特点,本研究采用核苷酸测序法检测了室内饲养种群及小菜蛾田间种群的击倒抗性基因频率,及氰戊菊酯处理后死亡个体的基因型组成。结果显示,本研究中仅检测到L1014F和T929I两个位点突变;室内饲养的小菜蛾2~4龄幼虫2个位点突变频率均为98.75%~100%,福建、浙江、内蒙古、北京等地小菜蛾田间种群在2个位点突变频率分别为90.00%~100%和88.75%~100%;2010-11~2011-11福州埔垱菜区田间小菜蛾2个位点突变频率相同,均为98.75%~100%;因氰戊菊酯处理死亡的个体在2个位点均为抗性纯合子。研究结果表明,小菜蛾抗性基因型较为单一;不同龄期、同一季节不同地区、同一地区不同季节小菜蛾抗性基因频率变化均较小;T929I突变在L1014F突变基础上产生,但当L1014F突变位点为杂合子时,T929I突变位点便为抗性杂合子或敏感纯合子;突变位点基因型不是小菜蛾对氰戊菊酯敏感性的唯一决定因素。本研究有助于更好了解小菜蛾对拟除虫菊酯类农药抗性的发生特点。
Plutella xylostella (Linn.), The most damaging pests of cruciferous vegetables, has generally developed severe resistance to pyrethroid pesticides. In order to understand the characteristics of the resistance of the diamondback moth to pyrethroid insecticides, the frequency of knockdown resistance genes in the indoor population and the diamondback moth in the population was detected by nucleotide sequencing, and the dead individuals after fenvalerate treatment The genotype composition. The results showed that only L1014F and T929I loci were detected in this study. Mutation frequency of two loci in 2nd and 4th instar larvae was 98.75% -100%, Fujian, Zhejiang, Inner Mongolia and Beijing The frequency of mutation in the diamondback moth from two sites was 90.00% -100% and 88.75% -100%, respectively. The frequencies of mutations in the two sites of the diamondback moth, Plutella xylostella, from 2010-11 to 2011-11 were the same Was 98.75% -100%. Individuals died of fenvalerate treatment were homozygous at 2 sites. The results showed that the resistant genotypes of Plutella xylostella were more single. The frequency of resistance genes of Plutella xylostella was less in different seasons and in the same season in different seasons. The T929I mutation was based on the L1014F mutation. However, when the L1014F mutation When heterozygous loci were detected, the T929I mutation sites were resistant heterozygotes or sensitive homozygotes; the mutation locus genotype was not the only determinant of sensitivity to fenvalerate in Plutella xylostella. This study helps to better understand the occurrence of pyrethroid insecticides in Plutella xylostella.