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目的探讨Ｐｉｌｏｃａｒｐｉｎｅ是否具有促使内耳分泌细胞功能亢进作用。方法 皮下注射Ｐｉｌｏ－ｃａｒｐｉｎｅ，用圆窗电极记录栗鼠ＣＭ，ＣＡＰ，ＳＰ，观察在用药前以及用药后３０’、６０’、９０’、１２０’、１５０’、１８０’检测栗鼠５００、１０００、２０００、４０００和８０００Ｈｚ的ＣＡＰ听阈，ＣＭ、ＳＰ振幅变化；实验结束后将动物处死，透射电镜下观察内耳形态学改变。结果皮下注射Ｐｉｌｏｃａｒｐｉｎｅ后１５’动物出现唾液分泌、排泄物增多的现象．３０’时所有频率的ＳＰ振幅增大，以 ２０００Ｈｚ最为显著，以后随着时间的延长，ＳＰ振幅逐渐增大，９０’后逐渐下降，１８０’时部分频率的ＳＰ振幅已恢复到用药前水平。在用药后各个频率的ＣＡＰ阈值的变化率呈正态分布，大多数动物无显著变化，只有少量动物的几个频率ＣＡＰ振幅在１０ｄＢ以内振荡，ＣＭ振幅无显著变化。透射电镜下内耳毛细胞形态正常，但在微纹区的暗细胞和内齿细胞（ｉｎｔｅｒｄｅｎｔａｌ ｃｅｌｌ， ＩＤＣ）内有着色较深的颗粒，并且部分颗粒向外分泌，在基底膜与盖膜间的空腔间形成层状物。结论 Ｐｉｌｏｃａｒｐｉｎｅ能促进内耳分泌功能，内耳的某些细胞（内齿细胞）有ｐｉｌｏｃａｒｐｉｎｅ受
Objective To investigate whether Pilocarpine promotes the function of secretory cells in inner ear. Methods Pilo-carpine was injected subcutaneously and the CM, CAP and SP were recorded by circular window electrodes. The chinchillas 500, 1000 and 2000 were detected before and 30, 60, 90, 120, 150 and 180 ’ , 4000, and 8000Hz CAP hearing threshold, CM, SP amplitude changes; after the end of the experiment the animals were sacrificed, observed under transmission electron microscope morphological changes of the inner ear. Results In the 15 ’animal after subcutaneous injection of Pilocarpine, salivary secretion and excretion increased. The amplitude of SP increased with the increase of time, and gradually decreased after 90 ’. At 180’, the SP amplitude of some frequency returned to the level before medication. After treatment, the rate of change of CAP threshold at each frequency showed a normal distribution, with no significant change in most animals. Only a few animals with several frequencies of CAP amplitude oscillated within 10 dB without significant changes in CM amplitude. Under the transmission electron microscope, the hair cells in the inner ear were normal in shape, but darker particles and darker granules were found in the dark cells and interdental cells (IDC) of the nodules, and part of the granules were excreted, leaving a void between the basement membrane and the lid membrane The intercavity forms a layer. Conclusion Pilocarpine can promote the secretion of the inner ear. Some of the inner ear cells (inner tooth cells) have pilocarpine