论文部分内容阅读
目的用隔离灌流的鼠肝硬变模型(IPCM)对硬变肝脏的循环顺应性进行观测。方法 CCl_4诱导的肝硬变大鼠60只分成6组,分别按基础流量及15、25、35、45、55ml/min 的不同灌流率行肝灌流,测量相应的血流动力学参数。结果与肝硬变对照组1.6±0.3kPa(16.8±2.9cmH_2O)的基础压力相比,35～55ml/min 的高灌流率伴有门静脉压力从2.5±0.4～3.9±0.7kPa(26±4～40±7cmH_2O)的迅速升高,灌流率的增大与门静脉压力的升高间有高度相关性(r=0.985;P<0.001),肝内阻力则变化不大(P>0.05)。IPCM 的最大稳定灌流范围为15～35ml/min,较文献报告的 IPRL 范围明显缩小。各组肝内阻力变化不大(P>0.05),门静脉压力的上升主要源于向肝血流量的增加,提示肝内血管床顺应性下降。结论 (1)肝硬变门静脉高压症时肝内循环顺应性下降而向肝血流量增加;(2)IPCM 是研究肝脏病、生理变化的好方法。
Objective To observe the circulatory compliance of cirrhotic liver with isolated perfused rat liver cirrhosis model (IPCM). Methods Sixty adult CCl_4-induced cirrhotic rats were divided into 6 groups. Hepatic perfusion was performed according to the basal flow rate and different perfusion rates of 15, 25, 35, 45 and 55ml / min respectively. The corresponding hemodynamic parameters were measured. Results Compared with the baseline pressure of 1.6 ± 0.3kPa (16.8 ± 2.9cmH 2 O) in cirrhotic control group, the high perfusion rate of 35 ~ 55ml / min was associated with portal pressure of 2.5 ± 0.4 ~ 3.9 ± 0.7kPa (26 ± 4 ~ 40 ± 7cmH2O). There was a significant correlation between the increase of perfusion rate and the increase of portal pressure (r = 0.985; P <0.001) and no significant change of intrahepatic resistance (P> 0.05). IPCM maximum steady perfusion range of 15 ~ 35ml / min, IPRL range reported in the literature significantly reduced. The changes of intrahepatic resistance in each group were not significant (P> 0.05). The increase of portal pressure was mainly due to the increase of hepatic blood flow, which suggested that the intrahepatic vascular bed compliance decreased. Conclusions (1) Liver cirrhosis portal hypertension with decreased hepatic circulation compliance and increased hepatic blood flow; (2) IPCM is a good way to study liver disease and physiological changes.