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各型肝炎中及献血者中TTV合并感染情况。设计特异性引物 ,采用半套式PCR法 ,对辽沈地区 2 0例散发性非甲 -非庚型肝炎 ,2 0例慢性乙型肝炎 ,2 0例慢性丙型肝炎及 40例重型肝炎与 5 0例献血者血清进行TTVDNA检测。非甲 -非庚型肝炎血清阳性 1例 ( 5 % ) ;重型肝炎阳性 9例 ( 2 2 .5 % ) ;慢性乙肝阳性 5例 ( 2 5 % ) ;慢性丙肝阳性 3例 ( 15 % ) ;献血者未查出TTVDNA阳性者。二株TTVDNA扩增产物的核苷酸序列分析与日本Nishizama基因同原性 98%以上。本地区献血者TTVDNA感染率极低 ;重型肝炎及慢性肝炎患者TTVDNA合并感染率较高 ,无输血史的散发性非甲 -非庚型肝炎中 ,血清TTVDNA阳性率为 5 % ,较其它报道输血后非甲 -非庚型肝炎的TTV感染率为低。
Various types of hepatitis and blood donors in the merger of TTV infection. Specific primers were designed and used to detect 20 cases of sporadic non-A-hepatitis E, 20 cases of chronic hepatitis B, 20 cases of chronic hepatitis C and 40 cases of severe hepatitis in Liaoning Shenyang area Fifty serum samples from donors were tested for TTVDNA. One case (5%) of non-A-HPG seropositivity; 9 cases (2.5%) of severe hepatitis; 5 cases (25%) of chronic hepatitis B positive; 3 cases (15%) of chronic hepatitis C positive; Blood donors did not detect TTVDNA positive. Nucleotide sequence analysis of the two TTVDNA amplification products was more than 98% homologous to the Japanese Nishizama gene. The prevalence of TTVDNA infection in blood donors in this area is very low. The prevalence of TTVDNA infection in patients with severe hepatitis and chronic hepatitis is high, and the positive rate of TTVDNA is 5% in sporadic non-A-non-G hepatitis without blood transfusion. Compared with other reported blood transfusions After non-A - non-hepatitis G TTV infection rate is low.