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对１１例重型肝炎病人乙型肝炎病毒（ＨＢＶ）ＤＮＡ直接序列进行分析以阐述变异特点。一次或套式ＰＣＲ扩增到的ＨＢＶＤＮＡ，以不对称ＰＣＲ法制备成单链模板后直接作序列分析。结果１１个病例中，９例有１～２个氨基酸替代变异：Ｇ１８９６→Ａ的替代，产生一个终止密码，见于１例抗－ＨＢｅ阳性的病人，第１７位（ＡＡ１７）缬氨酸被苯丙氨酸替代，见于１例ＨＢｅＡｇ阴性的病人，ＡＡ１５和ＡＡ２９的丝氨酸替代变异见于２例ＨＢｅＡｇ阴性、ＨＢＶ含量甚低的病例，１８５８Ｃ→Ｔ替代见于４例病人，与ＨＢｅＡｇ表型无关，无一伴有终２８变异，呈相互排斥现象。说明重型肝炎病人的ＨＢＶ前Ｃ基因存在多种变异类型。这些变异点只见于１～２例病人，似乎不是引发重型肝炎的重要原因，Ｇ１８９６→Ａ替代变异和ＡＡ１７的苯丙氨酸替代与ＨＢｅＡｇ阴性表型相关
Hepatitis B virus (HBV) DNA sequence analysis of 11 cases of severe hepatitis patients to explain the variation characteristics. HBVDNA amplified by one-time or nested PCR was directly sequenced after being prepared into single-stranded template by asymmetric PCR. Results Of the 11 cases, 9 cases had 1 ~ 2 amino acid substitution mutations: substitution of G1896 → A, resulting in a stop codon, seen in 1 anti-HBe positive patient, 17th position (AA17) Serine substitution in AA15 and AA29 found in 2 cases of HBeAg-negative, HBV content is very low, 1858C → T substitution seen in 4 patients, has nothing to do with the HBeAg phenotype The final 28 mutations, were mutually exclusive phenomenon. Description of hepatitis B patients with HBV pre-C gene there are multiple types of mutation. These mutations were found in only one or two patients and did not appear to be an important cause of severe hepatitis. Phenylalanine substitutions from G1896 → A and AA17 were associated with HBeAg-negative phenotype