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目的研究人γ-干扰素(human interferon-γ,huIFN-γ)基因修饰的结肠癌细胞瘤苗的成瘤性及抗肿瘤作用。方法用逆转录病毒质粒pL(IFN-γ)SN将人IFN-γ基因转导入鼠结肠癌细胞株CT26中,用G418进行筛选,得到抗性克隆CT26／IFN-γ,比较CT26／IFN-γ与野生型CT26的成瘤性,评价CT26／IFN-γ对野生型CT26细胞肝转移模型的治疗作用。结果CT26／IFN-γ能在含0．6 g·L-1 G418的培养液中稳定生长;RT-PCR证实IFN-γ基因在CT26／IFN-γ中表达;CT26／IFN-γ细胞能分泌人IFN-γ,体外培养体系中产量为3．5 fg·cell-1·d-1;CT26／IFN-γ的成瘤性比野生型CT26差[Balb／c小鼠肿瘤体积分别为(480±138)mm3与(991±176)mm3,F=31．29,P=0．000]; CT26／IFN-γ对野生型CT26的肝转移亦有明显抑制作用,荷瘤小鼠存活期明显延长(P=0．000)。结论人IFN-γ基因修饰的结肠癌细胞瘤苗有一定的抗肿瘤作用,值得进行深入研究。
Objective To study the tumorigenicity and antitumor effect of colon cancer cell vaccine modified by human interferon-γ (huIFN-γ) gene. Methods The human IFN-γ gene was transduced into the CT26 cell line of colon cancer using the retrovirus plasmid pL (IFN-γ) SN. The recombinant plasmid was screened by G418 to obtain the resistant clone CT26 / IFN-γ. CT26 / IFN- Tumorigenicity with wild-type CT26 to evaluate the therapeutic effect of CT26 / IFN-γ on the liver metastasis model of wild-type CT26 cells. Results CT26 / IFN-γ could stably grow in culture medium containing 0.6 g · L-1 G418. The expression of IFN-γ gene in CT26 / IFN-γ was confirmed by RT-PCR. CT26 / IFN- The tumorigenicity of CT26 / IFN-γ was lower than that of wild-type CT26 [tumor volume of Balb / c mice was (480 ± 138) mm3 and (991 ± 176) mm3 respectively, F = 31.29, P = 0.000]. CT26 / IFN-γ also significantly inhibited liver metastasis of wild-type CT26. Extended (P = 0.000). Conclusion Human IFN-γ gene modified colon cancer cell vaccine has certain anti-tumor effect, which deserves further study.