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[目的]探索清化肠饮对溃疡性结肠炎(UC)大鼠的治疗作用及其机制。[方法]将60只健康SPF级Wistar大鼠(雌雄各半),随机分成正常组,模型对照组,清化肠饮高、中、低剂量组和美沙拉秦组。除正常组外,其余各组均采用免疫复合法造模。观察大鼠疾病活动指数(DAI),ELISA法测定各组大鼠血清TNF-α、IL-6、IL-8及IL-10的变化情况。[结果]治疗前,正常组DAI与各造模组差异均有统计学意义(P<0.01);给药2周后,与模型对照组比较,清化肠饮高剂量组、美沙拉秦组大鼠显著降低(P<0.01)。从大鼠血清上看,与正常组比较,模型对照组大鼠TNF-α、IL-6及IL-8显著增高(P<0.01),而IL-10则显著降低(P<0.01);与模型对照组比较,清化肠饮高剂量组、美沙拉秦组大鼠TNF-α、IL-6及IL-8显著降低(P<0.01),IL-10则显著增高(P<0.01);与美沙拉秦组比较,清化肠饮中、低剂量组大鼠TNF-α、IL-6及IL-8显著增高(P<0.01),而IL-10则显著降低(P<0.01)。[结论]高剂量清化肠饮是治疗UC的有效药物,通过调节炎性细胞因子的平衡可能是其作用机制之一。
[Objective] To explore the therapeutic effect of Qinghua Changyin on ulcerative colitis (UC) rats and its mechanism. [Methods] Sixty healthy SPF Wistar rats (half male and female) were randomly divided into normal group, model control group, QingHuaZhengYin high, medium and low dose groups and mesalamine group. In addition to the normal group, the remaining groups were immunocompromised modeling. The disease activity index (DAI) of rats was observed. The changes of serum TNF-α, IL-6, IL-8 and IL-10 in serum of each group were measured by ELISA. [Results] Before treatment, the difference between DAI and each model group was statistically significant (P <0.01). After 2 weeks of administration, compared with the model control group, the high dose of Qinghuaiyin, mesalamine group Rats were significantly lower (P <0.01). Compared with the normal group, the levels of TNF-α, IL-6 and IL-8 in the model control group were significantly increased (P <0.01) and IL-10 was significantly decreased in the rat serum (P <0.01) Compared with the model control group, the levels of TNF-α, IL-6 and IL-8 were significantly decreased (P <0.01) and IL-10 was significantly increased (P <0.01) Compared with the mesalazine group, the levels of TNF-α, IL-6 and IL-8 in the Qinghuachangyin medium and low dose groups were significantly increased (P <0.01), while IL-10 was significantly decreased (P <0.01). [Conclusion] High-dose Qinghuachangyin is an effective drug for the treatment of UC. It may be one of its mechanisms through regulating the balance of inflammatory cytokines.