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Objective:To evaluate the effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs)and to explore the possible application on the alveolar bone regeneration.Methods:To determine the optimum concentration,the effects of ginsenoside Rg-1ranging from 10 to 100μmol/L were evaluated by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide,alkaline phosphatase activity and calcium deposition.Expressions of runt-related transcription factor 2,collagen alpha-2(l)chain,osteopontin,osteocalcin protein were examined using real-time polymerase chain reaction.Results:Compared with the control group,a certain concentration(10μmol/L)of the Rg-1 solution significantly enhanced the proliferation and osteogenic differentiation of hPDLSCs(P<0.05).However,concentrations that exceeds 100μmol/L led to cytotoxicity whereas concentrations below 10 nmol/L showed no significant effect as compared with the control.Conclusion:Ginsenoside Rg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10μmol/L.
Objective: To evaluate the effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and to explore the possible application on the alveolar bone regeneration. Methods: To determine the optimum concentration, the effects of ginsenoside Rg-1ranging from 10 to 100 μmol / L were evaluated by 3- (4,5) -dimethylthiahiazo (-z-y1) -3,5-di- phenytetrazoliumromide, alkaline phosphatase activity and calcium deposition. Expressions of runt-related transcription factor 2, collagen alpha-2 (l) chain, osteopontin, osteocalcin protein were examined using real-time polymerase chain reaction. Results: Compared with the control group, a certain concentration (10 μmol / L) of the Rg-1 solution significantly enhanced the Concentration and osteogenic differentiation of hPDLSCs (P <0.05) .However, concentrations that exceed 100 μmol / L led to cytotoxicity vs. concentrations below 10 nmol / L showed no significant effect compared with the control. Conlusion: Gins enoside Rg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10 μmol / L.