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研究了重组人肿瘤坏死因子α衍生物３ａ（ｒｅｃｏｍｂｉｎａｎｔｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒａｌｐｈａｄｅｒｉｖａｔｉｖｅ３ａ，ｒｈ－ＴＮＦαＤ３ａ）对多种肿瘤细胞株的体外直接杀伤作用。结果表明，ｒｈ－ＴＮＦαＤ３ａ在极低浓度下即能在体外对多种肿瘤细胞发挥明显的直接细胞毒作用，其对Ｌ９２９（小鼠成纤维细胞系），Ｅｈｒｌｉｃｈ（小鼠腹水癌细胞），Ｌｅｗｉｓ（小鼠肺癌细胞），ＳＭＭＣ７７２１（人肝细胞癌），Ｓ１８０（小鼠肉瘤细胞），Ｋ５６２（人红白血病细胞）和Ｕ９３７（人单核细胞性白血病细胞）的半数杀伤浓度分别为６．３３，８．０４，１６．４２，３８．２１，４３．２１，９６．２１和２７６．３１ｐｇ／ｍｌ。
The direct killing effect of recombinant human tumor necrosis factor alpha derivative 3a (rh-TNFαD3a) on various tumor cell lines was studied in vitro. The results showed that rh-TNFαD3a can exert a significant direct cytotoxic effect on a variety of tumor cells in vitro at a very low concentration, against L929 (mouse fibroblast cell line), Ehrlich (mouse ascites carcinoma cells), Lewis (Mice lung cancer cells), SMMC7721 (human hepatocellular carcinoma), S180 (mouse sarcoma cells), K562 (human erythroleukemia cells) and U937 (human mononuclear leukemia cells) half-kill concentrations were 6.33 , 8.04, 16.42, 38.21, 43.21, 96.21 and 276.31 pg/ml.