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目的观察抑癌基因ＭＴＳ２／ Ｐ１５及凋亡抑制基困Ｂｃｌ－２在低分化鼻咽癌（ＮＰＣ）中的表达，探讨其与低分化 ＮＰＣ发生、发展和预后的关系。方法用免疫组化 ＳＰ法检测 ４２例低分化 ＮＰＣ组织和门例无瘤鼻咽（ＮＰ）组织的Ｐ１５、Ｂｃｌ－２蛋白的表达情况。结果低分化ＮＰＣ中Ｐ１５、Ｒｃｌ－２蛋白的阳性表达率分别为５７．１％和８３．３％，ＮＰ组织中Ｐ１５蛋白表达率为１００％，而Ｂｃｌ－２蛋白大多在假复层纤毛柱状上皮的基底层细胞有表达，但较ＮＰＣ细胞染色弱，而在鳞状上皮多为阴性，可认为Ｂｃｌ－２蛋白在ＮＰ组织为阴性表达。Ｐ１５及Ｂｃｌ－２蛋白在ＮＰＣ与ＮＰ组中表达比较，两者差异有统计学意义（Ｐ＜０．０５）。Ｐ１５与Ｂｃｌ－２蛋白在ＮＰＣ中表达与临床分期、颈淋巴结转移、３年生存时间无关，其差异均无统计学意义（Ｐ＞０．０５）。两蛋白阳性表达之间无相关性。结论Ｐ１５蛋白在低分化ＮＰＣ中表达缺失和Ｂｃｌ－２蛋白在低分化ＮＰＣ中的过度表达对ＮＰＣ中的发生起一定作用。
Objective To observe the expression of tumor suppressor gene MTS2 / P15 and Bcl-2 in poorly differentiated nasopharyngeal carcinoma (NPC), and to explore its relationship with the occurrence, development and prognosis of poorly differentiated NPC. Methods Immunohistochemical SP method was used to detect the expression of P15 and Bcl-2 in 42 cases of poorly differentiated NPC tissues and tumor-free nasopharyngeal (NP) tissues. Results The positive expression rates of P15 and Rcl-2 in poorly differentiated NPC were 57.1% and 83.3%, respectively. The expression of P15 protein in NP tissues was 100%, while the expression of Bcl-2 protein was mostly in pseudostratified ciliated columnar Epidermal basal cells are expressed, but weaker than NPC cells staining, and mostly in the squamous epithelium is negative, Bcl-2 protein can be considered negative in NP tissue. P15 and Bcl-2 protein expression in NPC and NP group, the difference was statistically significant (P <0.05). The expression of P15 and Bcl-2 in NPC was not related to clinical stage, cervical lymph node metastasis and 3-year survival time (P> 0.05). No correlation between the two protein positive expression. Conclusion The lack of expression of P15 protein in poorly differentiated NPC and overexpression of Bcl-2 protein in poorly differentiated NPC may play a role in the pathogenesis of NPC.