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地方性氟中毒的神经系统受累现象日益引起重视,是非骨相损伤中最严重的一种。现认为氟引起中枢和周围神经受累除了压迫受损外还有氟对神经细胞的直接毒作用。为了证实此论点,我们用具有神经元特性的P_(C12)细胞株为模式,来检测氟对神经细胞的毒性,并用氟与镁、锌、硒3种元素分别或共同作用此细胞,以观察联合作用的效果。1 材料和方法 1.1 P_(C12)细胞培养物的制备此细胞从美国加州大学生物化学系获得。用RPMI_(1640)培养液(内含10％小牛血清,10％马血清,青、链霉素各100u/ml,PH7.0～7.2),37℃,5％CO_2培养,长成单层后待用。 1.2 氟、镁、锌、硒投毒液的制备氟化钠:上海试
Increasing attention has been paid to the neurological involvement of endemic fluorosis, the most serious of non-phantom injuries. Now that fluoride caused by the central and peripheral nerve involvement in addition to the impaired compression in addition to fluoride on the direct toxic effects of nerve cells. In order to confirm this argument, we used the P_ (C12) cell line with neuronal characteristics as a model to detect the toxicity of fluorine to nerve cells. The cells were treated with fluorine and magnesium, zinc and selenium separately or together to observe The effect of joint action. 1 Materials and Methods 1.1 Preparation of P_ (C12) Cell Cultures The cells were obtained from the University of California, Department of Biochemistry. The cells were grown in RPMI 1640 medium containing 10% fetal bovine serum, 10% horse serum, 100% fetal bovine serum and streptomycin, pH 7.0-7.2, grown at 37 ° C in 5% After use. 1.2 fluorine, magnesium, zinc, selenium preparation poisoning sodium fluoride: Shanghai test