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目的:研究选择性COX-2抑制剂塞来昔布诱导结肠癌细胞株HCT-116细胞周期阻滞的作用及其可能的机制。方法:应用流式细胞仪检测塞来昔布对HCT-116细胞周期的影响,定量PCR检测细胞周期素cyclinB1及COX-2 mRNA表达水平,Western-Blot检测细胞周期素cyclinB1的蛋白水平。结果:塞来昔布诱导HCT-116细胞G2/M阻滞的作用呈剂量依赖性,塞来昔布在mRNA及蛋白水平下调HCT-116细胞的cyclinB1。结论:塞来昔布能在体外抑制HCT-116细胞的增殖,诱导G2/M的阻滞,其作用与下调细胞周期素cyclinB1有关。
AIM: To investigate the role of selective COX-2 inhibitor celecoxib in inducing cell cycle arrest in human colon cancer cell line HCT-116 and its possible mechanism. Methods: The effect of celecoxib on the cell cycle of HCT-116 cells was detected by flow cytometry. The expression of cyclinB1 and COX-2 mRNA was detected by quantitative PCR. The protein level of cyclinB1 was detected by Western-Blot. Results: Celecoxib induced G2 / M arrest in HCT-116 cells in a dose-dependent manner. Celecoxib down-regulated cyclinB1 mRNA and protein in HCT-116 cells. CONCLUSION: Celecoxib can inhibit the proliferation of HCT-116 cells in vitro and induce G2 / M arrest, which is related to the down-regulation of cyclinB1.