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目的探讨胞外信号调节激酶(ERK)和核因子-κB(NF-κB)在胃腺癌及癌旁正常组织中的表达及其与临床病理参数之间的关系。方法通过免疫组化及RT-PCR法,检测36例胃腺癌组织及癌旁正常组织中ERK和NF-κB的蛋白及mRNA表达情况,分析组织中蛋白的表达率及表达强度与临床病理参数之间的关系。结果 (1)胃腺癌组织中ERK、NF-κB蛋白及mRNA表达显著高于癌旁正常组织(均P<0.01);(2)ERK及NF-κB在低分化、Ⅲ-Ⅳ期的胃腺癌组织中显著升高(均P<0.01),ERK在有淋巴结转移的胃腺癌组织中表达升高(P<0.05),而NF-κB在胃腺癌组织中的表达与淋巴结转移无明显的相关性(P>0.05),ERK及NF-κB在胃腺癌组织中的表达与患者的性别、年龄、病变部位亦无相关性(均P>0.05);(3)ERK和NF-κB的表达之间呈正相关(r=0.653,P<0.01)。结论胃腺癌组织中ERK和NF-κB表达上调,两者的表达成正相关。两者在胃癌的发生发展中可能起重要作用,为临床早期诊断、预后判断及治疗胃癌提供了新的思路。
Objective To investigate the expression of extracellular signal-regulated kinase (ERK) and nuclear factor-κB (NF-κB) in gastric adenocarcinoma and adjacent normal tissues and its relationship with clinicopathological parameters. Methods Immunohistochemistry and RT-PCR were used to detect the protein and mRNA expressions of ERK and NF-κB in 36 cases of gastric adenocarcinoma and adjacent normal tissues. The expression of protein and its expression intensity and clinicopathological parameters Relationship between. Results (1) The expressions of ERK and NF-κB protein and mRNA in gastric adenocarcinoma were significantly higher than those in adjacent normal tissues (all P <0.01). (2) The expression of ERK and NF-κB in poorly differentiated gastric adenocarcinoma (P <0.01). The expression of ERK in gastric adenocarcinoma with lymph node metastasis was increased (P <0.05), while the expression of NF-κB in gastric adenocarcinoma had no significant correlation with lymph node metastasis (P> 0.05). The expression of ERK and NF-κB in gastric adenocarcinoma had no correlation with the gender, age and lesion location (all P> 0.05). (3) The expression of ERK and NF- (R = 0.653, P <0.01). Conclusion The expression of ERK and NF-κB in gastric adenocarcinoma is up-regulated, and the expression of them is positively correlated. Both of them may play an important role in the occurrence and development of gastric cancer, providing a new idea for early clinical diagnosis, prognosis and treatment of gastric cancer.