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目的研究化疗药物对肺癌患者外周血中Treg(CD4+ CD25+调节性T细胞)的影响及意义。方法采集60例肺癌术后患者化疗前1天及化疗后第10天外周静脉血,应用流式细胞技术检测外周血中Treg细胞以及CD3+、CD4+、CD8+T、NK细胞占T淋巴细胞百分比,CD4+T/CD8+T比值。结果化疗前NSCLC患者外周血CD4+ CD25+调节T细胞比率明显高于健康对照组(P<0.05);且Ⅳ期患者调节T细胞比率明显高于Ⅲ期患者(P<0.05)。化疗后NSCLC患者外周血CD4+ CD25+调节T细胞较化疗前显著降低(P<0.05)。化疗前后不同病理分型患者外周血中CD4+CD25+细胞变化差异无统计学意义。化疗后CD8+T细胞占T淋巴细胞比例(28.129±10.900)%较化疗前(24.876±6.631)%升高(P<0.05)。化疗后CD4+/CD8+(1.506±0.691)较化疗前(1.680±0.704)降低(P<0.05)。结论肿瘤负荷可显著促进肺癌患者外周血Treg细胞分化,化疗后肺癌患者Treg细胞比例下降。
Objective To study the effect and significance of chemotherapeutic drugs on Treg (CD4 + CD25 + regulatory T cells) in peripheral blood of patients with lung cancer. Methods Peripheral venous blood was collected from 60 patients with postoperative lung cancer one day before chemotherapy and 10 days after chemotherapy. The percentage of T lymphocytes and the percentage of T lymphocytes, CD3 +, CD4 +, CD8 + T and NK cells in peripheral blood were measured by flow cytometry. CD4 + T / CD8 + T ratio. Results The percentage of CD4 + CD25 + regulatory T cells in peripheral blood of NSCLC patients before chemotherapy was significantly higher than that of healthy controls (P <0.05). The proportion of T cells in stage Ⅳ patients was significantly higher than that in stage Ⅲ patients (P <0.05). After chemotherapy, CD4 + CD25 + T cells in peripheral blood of NSCLC patients were significantly lower than those before chemotherapy (P <0.05). There was no significant difference of CD4 + CD25 + cells in peripheral blood between patients with different pathological types before and after chemotherapy. The percentage of CD8 + T cells in T lymphocytes (28.129 ± 10.900)% after chemotherapy was significantly higher than that before chemotherapy (24.876 ± 6.631)% (P <0.05). The ratio of CD4 + / CD8 + (1.506 ± 0.691) after chemotherapy was lower than that before chemotherapy (1.680 ± 0.704) (P <0.05). Conclusion Tumor burden can significantly promote the differentiation of Treg cells in peripheral blood of patients with lung cancer. The proportion of Treg cells in lung cancer patients after chemotherapy is decreased.