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[目的]探讨酒精性肝损伤时,枳黄方对内毒素信号通路中白细胞分化抗原14(CD14)表达的影响。[方法]将75只Wistar大鼠随机分为空白对照(正常)组、酒精攻击(模型)组、枳黄方(治疗)组,10 d后,处死大鼠,取大鼠血清和肝脏测定丙氨酸氨基转移酶(ALT)、天冬氨酸转氨酶(AST),苏木精-伊红染色观察肝脏病理变化,基质染色法测定血清内毒素,免疫组化法测定肝脏肿瘤坏死因子α(TNF-α),RT-PCR法测定肝组织CD14 mRNA的变化。[结果]治疗组肝脏病理表现较模型组好,其血清内毒素、ALT、AST和肝组织TNF-α、CD14 mRNA表达水平均高于正常组(P<0.05,<0.01,<0.01,<0.01,<0.05),且均显著低于模型组(均P<0.01)。[结论]枳黄方能显著降低内毒素信号转导通路上CD14基因水平的表达,减少肝组织TNF-α的表达,这可能是其对大鼠酒精性肝损伤有较明显保护作用的机制之一。
[Objective] To explore the effect of Qihuang Fang on the expression of leukocyte differentiation antigen 14 (CD14) in endotoxin signaling pathway during alcoholic liver injury. [Methods] Seventy-five Wistar rats were randomly divided into blank control (normal) group, alcohol challenge (model) group and Qihuangfang (treatment) group. After 10 days, the rats were sacrificed and the rats’ serum and liver were measured. Aminotransferase (ALT), aspartate aminotransferase (AST), hematoxylin-eosin staining were used to observe the pathological changes of the liver. Serum endotoxin was measured by stroma staining. Tumor necrosis factor alpha (TNF) was measured by immunohistochemistry. -α), RT-PCR method to determine the changes of liver tissue CD14. [Results] The liver pathology of the treatment group was better than that of the model group. The serum endotoxin, ALT, AST and liver tissue TNF-α, CD14 mRNA expression levels were higher than the normal group (P<0.05, <0.01, <0.01, <0.01 ,<0.05), and both were significantly lower than the model group (all P<0.01). [Conclusion] Radix Astragali can significantly reduce the expression of CD14 gene in endotoxin signal transduction pathway and reduce the expression of TNF-α in liver tissue, which may be one of the mechanisms of its protective effect on alcoholic liver injury in rats.