感染旋毛虫16～20周以后,宿主体内抗体消失,但幼虫仍存在,而ELISA法无法检测到阳性结果,所以旋毛虫特异性的DNA检测法就显得是一个更为准确的方法,尤其是在慢性感染的马群中。该实验用聚合酶链反应(PCR)进行检测。1.7kb的旋毛虫重复顺序DNA在基因组中很少分散,且呈串联状排列,有近2800个拷贝数,在2.5×10~8b After 16 to 20 weeks of infection with Trichinella, antibodies in the host disappeared, but the larvae were still present, whereas positive ELISA results were not detectable, so the Trichinella specific DNA assay appears to be a more accurate method, especially in the Among the chronically infected horses. The experiment was detected by polymerase chain reaction (PCR). The 1.7kb Trichinella repetitive DNA is sparsely distributed in the genome and arranged in tandem with nearly 2800 copies at 2.5x10-8b