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目的探讨HBV前S1抗原与HBeAg、HBV-DNA三者的相关性关系,分析PreS1Ag检测在判断乙型肝炎病毒复制中的作用。方法应用ELISA法对340份HBsAg阳性血清和100份健康体检(HBsAg阴性血清)进行PreS1-Ag和HBV血清标志物检测,并用实时荧光定量PCR检测HBV-DNA结果进行比较。结果在检测的340份HB-sAg阳性血清中,PreS1Ag阳性率为60.2%(205/340),HBeAg阳性率为43.5%(148/340),HBV-DNA阳性率为59.7%(203/340)。在148份HBeAg阳性血清中,PreS1Ag阳性率79.7%(118/148),HBV-DNA阳性率84.4%(125/148);192份HBeAg阴性血清中,PreS1Ag阳性率45.3%(87/192),HBV-DNA阳性率为40.6%(78/192)。HBeAg与HBV-DNA、PreS1的阳性率差异有统计学意义(P<0.05),HBeAg阳性血清中PreS1Ag(79.7%)阳性率显著高于HBeAg阴性血清PreS1Ag(45.3%)阳性率(P<0.01);PreS1抗原与HBV-DNA检出率有密切的相关性。340份HBsAg阳性血清中HBeAg、PreS1和HBV-DNA阳性数(率)分别为148(43.5%)、205(60.2%)、203(59.7%);100份HBsAg阴性血清中PreS1和HBV-DNA阳性数(率)分别为3(3%)、2(2%)。结论前S1抗原在乙肝诊断中是一个很重要的指标,它能够反映HBV复制的情况,PreS1Ag较HBeAg更敏感,两者比较差异有统计学意义(P<0.05)。前S1抗原与HBV-DNA阳性呈高度正相关。
Objective To investigate the relationship between HBV pre-S1 antigen and HBeAg and HBV-DNA and to analyze the role of PreS1Ag in judging hepatitis B virus replication. Methods PreS1-Ag and HBV serum markers were detected by ELISA in 340 HBsAg positive sera and 100 healthy volunteers (HBsAg-negative sera), and the results of HBV-DNA were compared by real-time fluorescence quantitative PCR. Results The positive rate of PreS1Ag was 60.2% (205/340), the positive rate of HBeAg was 43.5% (148/340) and the positive rate of HBV-DNA was 59.7% (203/340) in the 340 HBsAg positive sera. . PreS1Ag positive rate was 79.7% (118/148) and positive HBV-DNA rate was 84.4% (125/148) in 148 HBeAg positive sera. The positive rate of PreS1Ag in 192 HBeAg negative sera was 45.3% (87/192) The positive rate of HBV-DNA was 40.6% (78/192). The positive rate of PreS1Ag (79.7%) in HBeAg positive serum was significantly higher than that of PreS1Ag (45.3%) in HBeAg negative serum (P <0.01) ; PreS1 antigen and HBV-DNA detection rate is closely related. The positive numbers of HBeAg, PreS1 and HBV-DNA in 340 HBsAg positive sera were 148 (43.5%), 205 (60.2%) and 203 (59.7%) respectively. PreS1 and HBV-DNA were positive in 100 HBsAg negative sera Number (rate) were 3 (3%), 2 (2%). Conclusion Pre-S1 antigen is a very important indicator in the diagnosis of hepatitis B, which can reflect the situation of HBV replication. PreS1Ag is more sensitive than HBeAg, the difference between the two is statistically significant (P <0.05). Pre-S1 antigen was positively correlated with HBV-DNA positive.