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目的:研究泰山产白首乌醇提物对HepG2肝癌细胞增殖与凋亡的调节及其相关分子机制。方法:白首乌95%的乙醇提取物,通过D101大孔吸附树脂梯度洗脱。MTT法检测不同洗脱部位对HepG2细胞增殖的影响;流式细胞术检测HepG2细胞周期的变化;FITC-AnnexinⅤ/PI双标记检测90%乙醇洗脱部位对肝癌细胞凋亡的影响;免疫印迹法检测ERK,JNK1,p38/MAPK磷酸化水平的变化。结果:泰山白首乌50%乙醇洗脱部位和90%乙醇洗脱部位可明显抑制HepG2细胞增殖,减少细胞的增殖指数(PI),且90%乙醇洗脱部位可诱导HepG2细胞发生凋亡,抑制ERK磷酸化并促进JNK1的磷酸化。结论:泰山白首乌醇提物可抑制HepG2肝癌细胞的增殖并诱发凋亡,其分子机制可能与调节MAPK信号通路中ERK和JNK磷酸化水平的平衡相关。
OBJECTIVE: To study the regulation of the ethanol extract from Taishan Mountain on the proliferation and apoptosis of HepG2 hepatocarcinoma cells and the related molecular mechanisms. Method: Baishouwu 95% ethanol extract, through D101 macroporous resin gradient elution. The effect of different elution sites on the proliferation of HepG2 cells was detected by MTT assay. The changes of HepG2 cell cycle were detected by flow cytometry. The effect of 90% ethanol elution on the apoptosis of HepG2 cells was detected by FITC-Annexin Ⅴ / PI double- The changes of phosphorylation level of ERK, JNK1, p38 / MAPK were detected. Results: The elution fractions of 50% ethanol and 90% ethanol from the root of Radix et Rhizoma Rhei could inhibit the proliferation of HepG2 cells and decrease the proliferation index (PI) of HepG2 cells, and the apoptosis of HepG2 cells was induced by 90% ethanol elution. Inhibit ERK phosphorylation and promote phosphorylation of JNK1. Conclusion: Alcohol extract from Radix Et Rhizoma can inhibit the proliferation and induce apoptosis of HepG2 hepatocarcinoma cells. The molecular mechanism may be related to the balance of phosphorylation of ERK and JNK in MAPK signaling pathway.