用一步法直接单克隆抗体玫瑰花技术清除骨髓中的神经瘤细胞.两种MoAbs(Bw575,Bw625)可通过使用CrCl_3直接连接到牛红细胞上后,与神经瘤细胞形成玫瑰花特别强又稳定,再用密度梯度法分离出单个核细胞,并不影响骨髓干细胞生长,单个核细胞的回收率范围在35-69%之间,即使用了对骨髓有毒性的药物治疗的严重病人, One-step direct monoclonal antibody rose removal of neuroblastoma cells in the bone marrow.The two MoAbs (Bw575, Bw625) can be formed by the use of CrCl_3 directly on the bovine red blood cells, the formation of roses with neuroma cells is particularly strong and stable, Then using density gradient method to separate mononuclear cells, does not affect the growth of bone marrow stem cells, mononuclear cells in the range of 35-69% recovery, even with the use of bone marrow toxic drugs in patients with severe treatment,