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神经系统中5-HT受体分5-HT_1、5-HT_2和5-HT_3等不同类型,而5-HT_1受体又可分为5-HT_1A、5-HT_(1B)、5-HT_(1C)和5-HT_(1D)等亚型。分子生物学研究还证明,5-HT_1及5-HT_2受体属于与G蛋白有关的受体。本工作使用电压钳制技术第一次在豚鼠肠粘膜下神经丛神经元斑片膜上记录到由5-HT_3受体激活所产生的单离子通道电流及其电学特性,证实了5-HT_3受体与阳离子通道偶联,因此认为5-HT_3受体属递质门控离子通道。
The 5-HT receptors in the nervous system are classified into 5-HT 1, 5-HT 2 and 5-HT 3, and 5-HT 1 receptors can be divided into 5-HT 1 A, ) And 5-HT_ (1D) and other subtypes. Molecular biology studies have also shown that 5-HT 1 and 5-HT 2 receptors belong to the G protein-related receptors. In this work, voltage-clamp technique was used to record single ion channel currents and their electrical properties generated by the activation of 5-HT_3 receptor on guinea pig intestinal submucosal plexus neuronal patch cells for the first time. It was confirmed that 5-HT_3 receptor Coupled with the cation channel, it is believed that the 5-HT_3 receptor is a transmitter-gated ion channel.