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目的观察愈肾方对阿霉素(ADR)肾病模型大鼠的干预作用及其可能机制。方法 50只SD大鼠随机分为空白组、模型组、激素组、愈肾方大剂量组、愈肾方小剂量组,每组10只。除空白组外,其余各组大鼠均尾静脉注射2次(间隔7天)ADR建立肾病模型。末次注射后第3天,激素组给予醋酸泼尼松片9.0mg/kg灌胃,愈肾方大、小剂量组分别给予愈肾方39.6g/kg、19.8g/kg灌胃,空白组和模型组均给予等量饮用水,各组共灌胃21天。比较各组大鼠24h尿量及尿蛋白含量、肾功能、血脂、总蛋白(TP)、肾脏组织内皮素1(ET-1)及肾脏组织病理变化。结果与模型组比较,激素组和愈肾方大、小剂量组24 h尿量增加,愈肾方大、小剂量组尿蛋白含量减少(P<0.05或P<0.01)。与空白组比较,各组大鼠肾功能、血脂均显著增高,TP显著降低(P<0.05或P<0.01)。与空白组比较,模型组大鼠肾组织ET-1染色强度升高,激素组与模型组无显著差异,愈肾方大、小剂量组中ET-1染色强度降低。结论愈肾方可明显降低ADR肾病模型大鼠尿蛋白,可有效修复肾小球结构、足细胞及裂孔隔膜形态,其机理可能与减少ET-1的分泌有关。
Objective To observe the intervention effect of Yu Shen Fang on doxorubicin (ADR) nephropathy model rats and its possible mechanism. Methods Fifty Sprague-Dawley rats were randomly divided into blank group, model group, hormone group, high-dose Yueshenfang group and low-dose Yueshenfang group, with 10 rats in each group. Except for the blank group, all other rats in the other groups were injected with 2 doses of ADR (7 days apart) to establish the model of nephropathy. On the third day after the last injection, the hormone group was administered with prednisone acetate tablets 9.0mg / kg orally. The large and small dose groups were given 39.6g / kg and 19.8g / The model group were given the same amount of drinking water, each group a total of stomach 21 days. Urine volume and urinary protein, renal function, blood lipid, total protein (TP), endothelin-1 (ET-1) in renal tissue and histopathological changes in renal tissue were compared between the two groups. Results Compared with the model group, the urinary protein in 24 h in the hormone group and the Yushen Fang large and small dose groups increased, and the proteinuria of the large and small dose groups in the kidney reduced (P <0.05 or P <0.01). Compared with the blank group, the renal function, blood lipids were significantly increased in each group, TP was significantly decreased (P <0.05 or P <0.01). Compared with the blank group, the staining intensity of ET-1 in renal tissue of rats in model group was increased, and there was no significant difference between model group and hormone group. The staining intensity of ET-1 in large and small dose group was decreased. Conclusion Yu Shen can significantly reduce urinary protein in rats with ADR nephropathy, can effectively repair glomerular structure, podiatric and septal membrane morphology, the mechanism may be related to reducing the secretion of ET-1.