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目的探讨血管紧张素转化酶(ACE)基因插入/缺失(I/D)多态性与脑卒中的关系。方法用聚合酶链反应(PCR)方法观察比较131名脑卒中患者及与其年龄相匹配的健康人50名的ACE基因I/D多态性。在实验中插入特异引物进行二次PCR扩增,以避免纯合子DD基因型的误判。结果健康组对照、脑梗死组和脑出血组的基因型分布有差别(χ2=13.87,P=0.008),脑梗死组和脑出血组的DD基因型频率高于健康对照组的相应频率(χ2=9.21,P=0.002;χ2=8.76,P=0.003)。健康对照组、脑梗死组和脑出血组的D等位基因频率有差别(χ2=14.23,P=0.005),脑梗死组和脑出血组的D等位基因频率高于健康对照组的相应频率(χ2=11.17,P=0.001;χ2=10.87,P=0.001)。结论ACE基因具有I/D多态性,脑卒中与DD基因型和D等位基因有一定相关性,是可能引发脑卒中的危险遗传因素。
Objective To investigate the relationship between angiotensin converting enzyme (ACE) gene insertion / deletion (I / D) polymorphisms and stroke. Methods Polymerase chain reaction (PCR) method was used to compare ACE gene I / D polymorphism in 131 stroke patients and 50 matched healthy individuals. In the experiment, specific primers were inserted for secondary PCR amplification to avoid misjudgment of homozygous DD genotypes. Results There was a significant difference in genotype distribution between healthy controls and cerebral infarction patients and those with cerebral hemorrhage (χ2 = 13.87, P = 0.008). The frequency of DD genotype in cerebral infarction group and intracerebral hemorrhage group was higher than that in healthy control group (χ2 = = 9.21, P = 0.002; χ2 = 8.76, P = 0.003). The frequencies of D allele in cerebral infarction group and cerebral hemorrhage group were significantly different (χ2 = 14.23, P = 0.005). The frequency of D allele in cerebral infarction group and cerebral hemorrhage group was higher than that in healthy control group (χ2 = 11.17, P = 0.001; χ2 = 10.87, P = 0.001). Conclusions The ACE gene has I / D polymorphism. Stroke has some correlation with DD genotype and D allele, which is a risk genetic factor that may lead to stroke.