论文部分内容阅读
【目的】筛选分离可以分解玉米秸秆纤维素的菌株。【方法】采用平板稀释法从土样中分离纯化得到能够分解玉米秸秆纤维素的菌株,对分离得到的菌株进行生理生化及分子鉴定,同时以菌株Penicillium spp.(CICC40361)作为对照,比较菌株纤维素酶和木质素酶的活性,研究不同因素对菌株纤维素酶活力的影响,确定菌株纤维素酶动力学常数Km值。【结果】分离得到的菌株命名为PL2#,经生理生化及分子鉴定后,确定菌株PL2#为羊毛状青霉(Penicillium lanosum)。菌株PL2#的纤维素酶和木质素酶活力高于对照菌株Penicillium spp.;菌株PL2#的最优酶活测定条件为:1%CMC底物浓度,pH 4.8,50°C,酶反应时间60 min以及2 m L DNS添加量。【结论】羊毛状青霉(Penicillium lanosum)菌株PL2#比对照菌株Penicillium spp.具有更好地降解玉米秸秆纤维素的性能。
【Objective】 The objective of this study was to screen and isolate strains that could decompose corn stalk cellulose. 【Method】 The strains which could decompose corn stalk cellulose were isolated and purified from soil samples by plate dilution method. The physiological and biochemical and molecular identification of the isolated strains were carried out. At the same time, strain Penicillium spp. (CICC40361) Enzyme activity and ligninase activity, the effect of different factors on the cellulase activity of the strain was studied, and the kinetic Km value of the strain cellulase was determined. 【Result】 The isolated strain was named PL2 #. After biochemical and molecular identification, it was confirmed that strain PL2 # was Penicillium lanosum. The activity of cellulase and ligninase of strain PL2 # was higher than that of Penicillium spp .; the optimal enzyme activity of strain PL2 # was 1% CMC substrate concentration, pH 4.8, 50 ° C, enzyme reaction time 60 min and 2 m L DNS additions. 【Conclusion】 The Penicillium lanosum strain PL2 # has a better ability to degrade corn stalk cellulose than the control strain Penicillium spp.