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目的 :探讨Sebia HYDRASYS电泳系统在血清蛋白电泳中的应用。方法 :采用法国Sebia$CHY DRASYS电泳系统进行血清蛋白琼脂糖凝胶电泳 ,利用pH(8.8± 0 .1)Tris巴比妥缓冲液作电泳缓冲液 ,血清蛋白质根据其所带电荷数将其分离成ALB、α1、α2 、β、γ球蛋白 ,已分离的蛋白质由氨基黑染色 ,多余染色在酸性介质中被洗去 ,被分离的着色的蛋白质通过光密度仪扫描获得各区较为精确的相对值 ,并与醋酸纤维薄膜电泳法相比较。结果 :琼脂糖凝胶电泳法批内精密度 (CV)为 0 .72 % (白蛋白 )至 4 .98% (α1-球蛋白 )之间 ,总精密度 (CV)为 2 .5 2 % (白蛋白 )至 6 .0 3% (γ球蛋白 )之间 ,较醋酸纤维薄膜电泳法显示较好的精密度 ,琼脂糖凝胶电泳还显示出独特的区带扫描特征。结论 :Sebia-HYDRASYS电泳系统可显示出独特的区带扫描特征 ,分辨率高 ,精密度好 ,简便快速等优点。
Objective: To investigate the application of Sebia HYDRASYS electrophoresis system in serum protein electrophoresis. Methods: Serum protein agarose gel electrophoresis was carried out by the Sebia $ CHY DRASYS electrophoresis system in France. Tris (8.8 ± 0.1) Tris-barbital buffer was used as electrophoresis buffer. The serum protein was separated according to the number of charged ions Into ALB, α1, α2, β, γ globulin, the protein has been isolated by the amino black staining, redundant staining was washed away in acidic medium, the colored protein was isolated by scanning densitometer relative accuracy of each region , And compared with cellulose acetate film electrophoresis. Results: The intra-assay precision (CV) of agarose gel electrophoresis was 0.72% (albumin) to 4.98% (α1-globulin) with a total precision (CV) of 2.52% (Albumin) to 6.03% (gamma globulin), showing better precision than acetate film electrophoresis, and agarose gel electrophoresis also showed unique zone-scanning characteristics. Conclusion: Sebia-HYDRASYS electrophoresis system can display unique zone scanning features, high resolution, good precision, simple and quick and so on.