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为初步明确大豆田反枝苋对咪唑乙烟酸的抗药性水平,并从分子角度对抗药性机制进行解释,以我国四川成都和黑龙江嫩江采集的反枝苋种子为材料,通过琼脂法检测了反枝苋对咪唑乙烟酸的抗药性水平,并分别对R(嫩江抗性种群)和S(成都敏感种群)的乙酰乳酸合成酶(ALS)部分序列进行扩增和测序。皿内生测结果表明,成都种群的GI50为11.20,嫩江种群的GI50为52.26,其抗药性指数RI为4.67。分子检测结果表明,与S种群相比,R种群反枝苋ALS位于高度保守区Domain B编码574位氨基酸的基因发生突变,TGG突变为TTG,导致色氨酸被亮氨酸取代。ALS保守区域氨基酸的替换可能是嫩江反枝苋种群对咪唑乙烟酸产生抗性的重要原因之一。
In order to clarify the drug resistance of imidacloprid in soybean field, and to explain the mechanism of drug resistance from molecular point of view, the anti-Amaranth were collected from Chengdu, Sichuan, China and Nenjiang, Heilongjiang by Agar. , And the partial sequences of acetolactate synthase (ALS) of R (Nenjiang resistant population) and S (sensitive population of Chengdu) were amplified and sequenced respectively. The results of bioassay showed that the GI50 of Chengdu was 11.20, that of Nenjiang was 52.26, and its resistance index RI was 4.67. The results of molecular analysis showed that compared with the S population, the mutation of the gene of 574 amino acids in Domain B of the R population of Amaranthus retroflexus Amaranth in the highly conserved region resulted in the mutation of TTG to TTG, resulting in the replacement of tryptophan by leucine. The substitution of amino acids in the conserved region of ALS may be one of the important reasons for the resistance of imidacloprid to the population of Nenjiang Amaranthus retroflexus.