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在兔肌腱细胞、成纤维细胞分离培养的基础上,将冻存复苏的传代细胞与碳纤维、涤纶及几丁质三种材料体外联合培养,观察细胞与材料的相容性以及细胞在材料上的生长情况,比较这两种细胞在三种材料上的生长差异。结果发现,在体外培养条件下,碳纤维与两种细胞的相容性均好,有良好的吸附性;涤纶材料上两种细胞生长均少,可能与其表面结构有关;几丁质明显抑制这两种细胞生长。同时发现,无论肌腱细胞,还是成纤维细胞,在碳纤维上座落的数量都明显优于涤纶、几丁质。三种材料交错在一起时,胶原纤维方向是以碳纤维为轴,形成网状,交织缠绕在材料之间。随时间延长,细胞在单根碳纤维或多根碳纤维上排列均匀,包绕碳纤维,且相互衔接。研究结果,为进一步研究有生命的人工肌腱、韧带提供了依据。
Based on the isolation and culture of rabbit tendon cells and fibroblasts, the frozen-thawed passage cells were cultured in vitro with carbon fiber, polyester and chitin to observe the compatibility of cells and materials and the cell-to-cell Growth, compare the growth difference of these two kinds of cells in three kinds of materials. The results showed that under the conditions of in vitro culture, the compatibility of carbon fiber with the two kinds of cells was good and good adsorption. The two kinds of cells on the polyester material were less growth, which may be related to its surface structure; chitin obviously inhibited both Cell growth. Also found that both tendon cells, or fibroblasts, the number of carbon fiber are located on the significantly better than polyester, chitin. When the three kinds of materials are staggered together, the direction of the collagen fibers is the carbon fiber axis, forming a network, interweaving entangled between the materials. Over time, the cells are arranged on a single carbon fiber or multiple carbon fibers uniformly, wrapping the carbon fiber and connecting with each other. The results provide the basis for further study of living artificial tendons and ligaments.