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食管癌细胞(EC9706)经不同剂量X射线和60Coγ射线辐照后继续培养24h,利用激光拉曼光谱分析EC9706细胞内部蛋白质、核酸、脂类等生物大分子的构象和含量变化。分析发现,两种放射源各剂量组拉曼光谱的峰强和频移与空白对照组之间都存在较大的差异。主要表现为(1)X射线辐照后,对照组光谱中存在的某些谱带,个别剂量组中该谱带却消失。蛋白质主链中骨架C-N振动引起的1114 cm-1谱带在各剂量组中普遍消失,膜脂中膜结合β-胡萝卜素的C=C振动谱带1523 cm-1仅存在于6Gy组。(2)60Coγ射线辐照后,蛋白质酰胺Ⅲ带在中等剂量(4、5Gy)组中β折叠结构向无规卷曲转化,大剂量组中DNA中的磷酸二酯(O-P-O)基团的非氢键化程度增强。拉曼特征峰在不同剂量组中的变化,为进一步从物理能级结构角度研究X射线和60Coγ射线对EC9706细胞的辐照损伤机制提供了实验依据。
Esophageal cancer cells (EC9706) were irradiated with different doses of X-rays and 60Coγ-rays for 24 hours. The conformations and contents of biomacromolecules such as proteins, nucleic acids and lipids in EC9706 cells were analyzed by laser Raman spectroscopy. Analysis showed that there was a big difference between the peak intensity and the frequency shift of Raman spectra of the two radiation sources in each dose group and the blank control group. Mainly as follows: (1) X-ray irradiation, the control group of the spectrum of some of the bands, the individual dose group disappeared. The 1114 cm-1 band caused by the skeleton C-N vibration in protein backbone disappeared in all dose groups. The C = C vibrational band of membrane-bound β-carotene at 1523 cm-1 existed only in 6Gy group. (2) After irradiation with 60Coγ-ray, the protein amide Ⅲ band converted to random coil in middle-dose (4,5Gy) group, but the non-hydrogen of OPO group The degree of bond enhancement. Raman peak in different dose groups, which provide experimental evidence for further study on the mechanism of radiation damage of EC9706 cells by X-ray and 60Coγ-ray from the perspective of physical level structure.