目的了解医疗操作中医护人员手污染程度及洗手方法的应用情况。方法采用现场涂抹采样方法和活菌数检验方法进行了检测。结果该医院医护人员在医疗操作前,手上平均带菌数为51cfu/cm2,在诊疗过程中手上带菌数平均为32cfu/cm2。用普通肥皂洗手后,手上带菌数平均为24cfu/cm2;用含异丙醇的快速手消毒液擦手后,手上带菌数平均为4cfu/cm2。结论医护人员手在没有经过清洗或消毒的情况下,手上带菌数超标明显;用普通肥皂洗手对菌数降低不明显,用快速手消毒剂擦手消毒效果较好。 Objective To understand the degree of hand contamination of medical staff and the application of hand washing methods in medical operations. Methods The methods of on-site smear sampling and viable count test were used. Results Before the medical operation, the average number of bacteria in the hand was 51cfu / cm2. The average number of bacteria carried in the hand during the treatment was 32cfu / cm2. After washing hands with ordinary soap, the average number of bacteria in the hands was 24cfu / cm2; after the hand-washing with isopropyl alcohol-containing fast hand disinfectant, the average number of bacteria in the hands was 4cfu / cm2. Conclusion The number of hand-held bacteria exceeded the standard obviously when the hand of medical staff was not cleaned or disinfected. The hand-washing with ordinary soap did not obviously reduce the number of bacteria, and hand-disinfecting with hand-disinfecting agent with fast hand was better.