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目的:观察不同浓度的三氧化二砷作用不同时间对兔晶体上皮细胞影响及其作用机理。方法:应用不同浓度的三氧化二砷作用于兔晶体上皮细胞后,观察三氧化二砷对兔晶体上皮细胞生长状态的影响;用噻唑蓝(MTT)比色法、透射电镜技术观察其对兔晶体上皮细胞增殖的影响;应用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)及流式细胞技术检测三氧化二砷对兔晶体上皮细胞凋亡的诱导作用。结果:不同浓度的三氧化二砷作用于兔晶体上皮细胞有明显的时间和剂量依赖性。三氧化二砷作用后细胞生长明显受抑制,并出现明显的凋亡特征性改变;8μmol/L三氧化二砷作用24小时、48小时及72小时后TUNEL法可检测到细胞凋亡水平显著性升高,流式细胞仪分析显示,兔晶体上皮细胞在G1峰前出现明显的凋亡峰。结论:三氧化二砷可明显抑制兔晶体上皮细胞的生长,其机理主要是诱导兔晶体上皮细胞凋亡。
Objective: To observe the effect of different concentrations of arsenic trioxide on rabbit lens epithelial cells at different times and its mechanism. Methods: The effects of arsenic trioxide on the growth of rabbit lens epithelial cells were observed by using different concentrations of arsenic trioxide on the growth of rabbit lens epithelial cells. The effects of arsenic trioxide on the growth of rabbit lens epithelial cells were observed by MTT assay and transmission electron microscopy The apoptosis of lens epithelial cells was detected by TUNEL and flow cytometry. Results: Different concentrations of arsenic trioxide on the rabbit lens epithelial cells in a significant time and dose-dependent manner. After arsenic trioxide treatment, the cell growth was significantly inhibited, and the obvious characteristic of apoptosis was observed. Apoptosis level was significantly increased by TUNEL after treated with 8μmol / L arsenic trioxide for 24 hours, 48 hours and 72 hours. Flow cytometry Instrument analysis showed that rabbit crystal epithelial cells in the G1 peak before significant apoptotic peak. Conclusion: Arsenic trioxide can significantly inhibit the growth of rabbit lens epithelial cells, and its mechanism is mainly to induce the apoptosis of rabbit lens epithelial cells.