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目的 :探讨不同压力状态对动脉平滑肌增殖的调控作用 ,由此判断冠脉再狭窄过程中压力因素所起的作用。方法 :用含 10 %小牛血清的RPMI- 16 40培养液体外培养新西兰白兔的主动脉平滑肌细胞至 3～ 7代 ,将含 5 %小牛血清的RPMI- 16 40培养液与动脉平滑肌细胞的混悬液 10 0 μl加入 96孔培养板中培养。 2 4h后按照实验分组分别在 6 .6 5kPa ,13.3kPa,19.95kPa ,2 6 .6kPa压力状态下持续培养 48h ,采用四唑盐比色法和3 H -TdR参入法检测平滑肌的生长状况。结果 :13.3kPa压力状态对兔主动脉平滑肌细胞有显著的促增殖作用 (P <0 .0 5 ) ;6 .6 5kPa,19.95kPa ,2 6 .6kPa压力状态对兔主动脉平滑肌细胞无显著的促增殖作用 (P >0 .0 5 )。结论 :生理血压的 1.5～ 2倍压力状态对兔动脉平滑肌细胞增殖有显著的促进作用。
OBJECTIVE: To investigate the regulation of arterial smooth muscle cell proliferation under different stress states, and to determine the role of stress factors in coronary restenosis. Methods: New Zealand white rabbits aorta smooth muscle cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum for 3-7 generations. RPMI-1640 medium containing 5% fetal calf serum and arterial smooth muscle cells 100 μl of the suspension was added to a 96-well culture plate. After 24 hours, the cells were cultured for 48 hours under the pressure of 6. 5 kPa, 13.3 kPa, 19.95 kPa and 26.6 kPa, respectively. The growth of smooth muscle was detected by tetrazolium salt method and 3 H-TdR method. Results: The pressure of 13.3kPa significantly promoted the proliferation of rabbit aortic smooth muscle cells (P <0.05); 6. 6kPa, 19.95kPa, 26kPa pressure on rabbit aortic smooth muscle cells showed no significant Promote proliferation (P> 0.05). Conclusion: The physiological pressure of 1.5 ~ 2 times the pressure state of rabbit artery smooth muscle cell proliferation was significantly promoted.