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研究了具有抗HIV 1活性的大环多胺类化合物与RNA的识别作用 ,以及其对cos 7细胞凋亡的影响 ,以进一步探讨其抗HIV 1的作用机理 .实验采用琼脂糖凝胶电泳方法 ,观察化合物与RNA的识别作用 ;通过流式细胞计数法探讨其对cos 7细胞凋亡的影响 ;运用计算机分子模型 ,从理论上Docking计算化合物与TARRNA结合的可能性 .结果表明 ,大环多胺类化合物MP 1、MP 2和MP 3不仅具有断裂RNA的作用 ,并可抑制Tat RNA的相互作用 ,还可影响cos 7细胞亚二倍体的含量 ;理论化学计算数据与实验结果基本一致 .这一结果提示化合物的抗HIV 1活性可能通过作用于病毒基因组RNA而发挥作用 ,是多靶作用的结果 .
To study the recognition of macrocyclic polyamines with anti-HIV 1 activity on RNA and its effect on cos 7 cell apoptosis in order to further explore its mechanism of anti-HIV 1. Experiments using agarose gel electrophoresis , And observed the compound and RNA recognition role; Through flow cytometry to explore the cos 7 cell apoptosis; using computer molecular model, theoretically Docking calculation compound and TARRNA binding possibility.The results showed that the macrocyclic The amines MP 1, MP 2 and MP 3 not only have the effect of cleaving RNA, but also inhibit the interaction of Tat RNA and the sub-diploid content of cos 7 cells. The theoretical stoichiometric data are consistent with the experimental results. This result suggests that the anti-HIV 1 activity of compounds may play a role by acting on viral genomic RNA as a result of multi-target effects.