论文部分内容阅读
目的探讨大剂量高压氧治疗方案对永久性大脑中动脉闭塞大鼠的疗效以及高压氧对大鼠梗死部位周围脑组织核因子κB(nuclear factor-κB,NF-κB)影响。方法制备雄性Sprague-Dawley大鼠永久性大脑中动脉闭塞模型,随机分为高压氧组和对照组,每组32只,另设立伪手术组。使用Garcia神经行为学评分方法分别在术后24h、5d对大鼠进行神经行为学评分;应用2,3,5-三苯基氯化四氮唑(2,3,5-triphenyltetrazolium chlorid,TTC)方法对脑组织进行染色,观察24h、5d时大鼠脑组织梗死容积;取梗死部位周围脑组织,采用凝胶电泳迁移实验(electrophoretic mobility shift assay,EMSA)方法检测术后24h、5d时的NF-κB脱氧核糖核酸(deoxyribonucleic acid,DNA)结合活性。比较三组间上述指标的差异。结果术后24h高压氧组神经行为学评分高于对照组[(13.33±1.53)vs(10.33±0.58),P<0.001]。术后24h高压氧组梗死容积小于对照组[(139.73±33.59)vs(203.02±57.66),P=0.008]。术后5d高压氧组梗死部位周围脑组织NF-κB活性低于对照组[(16.01±4.56)vs(50.28±9.13),P=0.035]。结论大剂量高压氧治疗方案在脑梗死后24h内具有脑保护作用。大剂量高压氧可降低大鼠梗死部位周围脑组织NF-κBDNA结合活性。
Objective To investigate the therapeutic effect of high-dose hyperbaric oxygen therapy on permanent middle cerebral artery occlusion in rats and the effect of hyperbaric oxygen on nuclear factor-κB (NF-κB) in brain tissue around the infarcted area of rats. Methods Permanent middle cerebral artery occlusion model of male Sprague-Dawley rats were randomly divided into hyperbaric oxygen group and control group, with 32 rats in each group. A pseudo-surgery group was also established. Neurobehavioral score of Garcia was used to evaluate neurological behavior at 24h and 5d postoperatively respectively. 2,3,5-triphenyltetrazolium chloride (TTC) Methods Brain tissue was stained to observe infarct volume at 24h and 5d. Brain tissue was collected from the infarcted area and electrophoretic mobility shift assay (EMSA) was used to detect NF -κB deoxyribonucleic acid (DNA) binding activity. Compare the difference between the above three indicators. Results The neurobehavioral scores of hyperbaric oxygen group after operation were significantly higher than those of the control group [(13.33 ± 1.53) vs (10.33 ± 0.58), P <0.001]. The infarct volume of hyperbaric oxygen group after operation was less than that of the control group [(139.73 ± 33.59) vs (203.02 ± 57.66), P = 0.008]. The activity of NF-κB in brain tissue around the infarction site in the hyperbaric oxygen group was lower than that in the control group 5 days after operation ([(16.01 ± 4.56) vs (50.28 ± 9.13), P = 0.035]. Conclusion High-dose hyperbaric oxygen therapy has brain protection effect within 24h after cerebral infarction. High-dose hyperbaric oxygen can reduce NF-κB DNA binding activity in brain tissue around the infarct of rats.